PHYLOGENY OF ASTRAGALUS SECTION DISSITIFLORI BASED ON nrDNA ITS AND MORPHOLOGICAL DATA IN IRAN

Document Type : Research Paper

Authors

1 PhD student/Faculty of Biological Sciences, Shahid Beheshti University G. C., Tehran

2 Associate Professor/Faculty of Energy Engineering and New Technologies, Shahid Beheshti University G. C., Tehran

3 Associate Professor/Faculty of Biological Sciences, Tarbiat Modares University

4 Professor/Botany Division, Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran

Abstract

Twenty four species belonging mainly to Astragalus L. section Dissitiflori DC. and some related sections were analyzed using maximum parsimony and Bayesian methods on the basis of nrDNA ITS sequences and morphological data. Trees resulted from two datasets were in agreement on their overall topologies. Based on our results, members of the section Dissitiflori didn't constitute a monophyletic group. However, the section with inclusion of the species from the other related ones, considered to be a monophyletic group. Since A. virgatus as the lectotype of the section Dissitiflori was not included in the present study; it is difficult to evaluate the monophyly of the section and to delimit it explicitly. Astragalus juladakensis, shows a basal position in both nrDNA ITS and the combined nrDNA ITS-morphology trees, as a sister to the remaining species of the sect. Dissitiflori. However, according to the present study, the affinity of this species to the section appeared to be questionable. Based on our molecular data, three species belonging to the section Corethrum in Iran, are pertained to the sect. Dissitiflori. In addition, our results revealed that A. pravitzii, which had been transferred to sect. Ornithopodium Bunge, belongs to the section Dissitiflori.

Keywords

Article Title [Persian]

فیلوژنی بخشه Dissitiflori از جنس گون در ایران براساس داده‌های ریخت شناختی و nrDNA ITS

Authors [Persian]

  • رضا شیخ اکبری مهر 1
  • عباس سعیدی 2
  • شاهرخ کاظم پور اصالو 3
  • علی اصغر معصومی 4
1 دانشجوی دکترا/دانشکده علوم زیستی، دانشگاه شهید بهشتی، تهران
2 دانشیار/ دانشکده مهندسی انرژی و فناوریهای نوین، دانشگاه شهید بهشتی، تهران
3 دانشیار/دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران
4 استاد/بخش گیاهشناسی، موسسه تحقیقات جنگلها و مراتع کشور، تهران
Abstract [Persian]

در مطالعه حاضر فیلوژنی بیست و دو گونه از گون‌های کرک‌ دوشاخه‌ای متعلق به بخشه دیسیتی فلوری و چند بخشه وابسته و نزدیک به آن، با استفاده از داده‌های ریخت‌شناختی و مولکولی مورد ارزیابی قرار گرفت. براساس نتایج بدست آمده، بخشه Dissitiflori در تمام آنالیزهای انجام ‌شده، به تنهایی گروهی منوفیلتیک را تشکیل نمی‌دهد. البته اعضای این بخشه بهمراه گونه‌های مربوط به دیگر بخشه‌های نزدیک به آن را می‌توان یک گروه تک‌تبار در نظر گرفت. بنظر می‌رسد برای تعیین دقیق وضعیت فیلوژنتیکی بخشه Dissitiflori بایستی مطالعات بیشتر با استفاده از قطعات دیگری از ژنوم و همچنین وارد کردن گونهA. virgatus. (بعنوان لکتوتیپ بخشه Dissitiflori) در آنالیزها، انجام پذیرد. براساس نتایج حاصل از داده‌های دی اِن اِی ریبوزومی، گونه A. juladakensis یک گروه خواهری را نسبت به بقیه گونه‌های مطالعه‌شده تشکیل داد. بنظر می‌رسد جایگاه این گونه بایستی در بخشه دیگری از جنس ارزیابی شود. همچنین نتایج مطالعه مولکولی (براساس دی اِن اِی ریبوزومی) و ریخت شناختی نشان دادند، گونه A. pravitzii که به بخشه Ornithopodium منتقل شده بود به Dissitiflori تعلق دارد.

Keywords [Persian]

  • Astragalus sect. Dissitiflori
  • Bayesian Method
  • Morphology
  • nrDNA ITS
  • parsimony
  • phylogeny

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History

  • Receive Date: 19 October 2011
  • Revise Date: 22 January 2012
  • Accept Date: 21 February 2012

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