Chromosome counts report of six Astragalus L. (Fabaceae) species from Iran

Document Type : Research Paper


1 Forests and Rangelands Research Department, Kermanshah Agricultural and Natural Resources Research and Education Center, (AREEO), Kermanshah, Iran

2 Natural Resources Division, Fars Agricultural, and Natural Resources Research and Education Centre, (AREEO), Shiraz, Iran

3 Forests and Rangelands Research Division, Kermanshah Agricultural and Natural Resources Research and Education Center, (AREEO), Kermanshah, Iran.

4 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.


Somatic chromosome numbers of six endemic and rare species of the genus Astragalus were studied. The basic chromosome number for all studied species was x = 8. Five of the studied species including A. assadabadensis, A. carduchorum, A. kirrindicus, A. nervistipulus, and A. microcephalus subsp. pycnocladus were diploid and A. trachyacanthos was tetraploid. The chromosomal parameters of the species were calculated and their idiograms were drawn. The karyotypic formula of the species was different, but in all species most of the chromosomes were metacentric and there were zero to three submetacentric chromosomes. Astragalus assadabadensis had the longest chromosomes and A. trachyacanthos had the shortest chromosomes. Chromosome count of three species including A. carduchorum from the sect. Acidodes, A. microcephalus subsp. pycnocladus and A. trachyacanthos from the sect. Rhacophorus are reported for the first time.


Article Title [Persian]

شمارش کروموزومی شش گونه از جنس Astragalus (Fabaceae) از ایران

Authors [Persian]

  • نسترن جلیلیان 1
  • سارا صادقیان 2
  • هوشمند صفری 3
  • عادل جلیلی 4
  • فرشته اسدی‌کرم 4
1 استادیار پژوهش بخش تحقیقات جنگلها و مراتع، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، سازمان تحقیقات، آموزش و
2 کارشناس پژوهش بخش تحقیقات منابع طبیعی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان فارس، سازمان تحقیقات، آموزش و ترویج کشاورزی، شیراز، ایران
3 استادیار پژوهش، بخش تحقیقات جنگلها و مراتع، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمانشاه، ایران
4 استاد پژوهش، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
Abstract [Persian]

عدد کروموزومی 6 گونه انحصاری و نادر از جنس گون (Astragalus) در این تحقیق بررسی شد. عدد پایه کروموزومی برای تمام گونه‌ها 8=x بود. گونه‌های A. assadabadensis، A. carduchorum، A. kirrindicus، A. nervistipulus وA. microcephalus subsp. pycnocladus دیپلوئید و گونه‌ی A. trachyacanthos تتراپلوئید بود. پارامتر‌های کروموزومی گونه‌ها بررسی شد و ایدیوگرام آنها رسم گردید. فرمول کاریوتیپی گونه‌ها متفاوت بود اما در تمام آنها بیشتر کروموزوم‌ها متاسنتریک بودند و تعداد صفر تا 3 کروموزوم ساب متاسنتریک در فرمول کاریوتیپی وجود داشت. گونه‌ی A. assadabadensis بلندترین طول کروموزوم‌ها و گونه‌ی A. trachyacanthos کوتاه‌ترین طول کروموزوم‌ها را داشتند. عدد کروموزومی گونه‌ی A. carduchorum از بخش Acidodes، گونه‌ی A. microcephalus subsp. pycnocladus وA. trachyacanthos  از بخش Rhacophorus برای اولین بار گزارش می‌گردد.

Keywords [Persian]

  • Astragalus
  • chromosome counts
  • Fabaceae
  • idiograms
  • karyotype
  • Iran
Akhavan Roofigar, A. & Jalili, A. 2021: Chromosome numbers report of five endemic Astragalus L. (Fabaceae) species of Iran. -Iran. J. Bot. 27 (2): 177-181.
Aryavand, A. 1977: IOPB chromosome number reports LVII. -Taxon 26: 443-452.
Aryavand, A. 1983: IOPB Chromosome Number Reports LXXX. -Taxon 32: 504-511.
Badr, A., Hamoud, M., El-Rabey, H. 1996: Chromosomal studies in the Egyptian flora V. Chromosomal relationship in the genus Astragalus L. (Fabaceae) and their taxonomic inferences.  -Cytologia 61 (1):105-111.
Bagheri, A., Akhavan Roofigar, A., Nemati, Z. & Blattner, F. R. 2022: Genome size and chromosome number evaluation of Astragalus L. sect. Hymenostegis Bunge (Fabaceae).-Plants. 11.435.
Cartier D., 1979 -Premieres prospections caryologiques du genre Astragalus L. dans la flore du basin mediterraneen oriental. Revue de Cytologie et de Biologie Végétales, le Botaniste, 2: 169-181.
Ghaffari, S. M. 2020: Index to plant chromosome number of Iran. Research Institute of Forests and Rangeland. Tehran, Iran. 336 pp.
Hesamzadeh Hejazi, S.M. & Ziaei Nasab, M. 2009: Color chromosome atlas of Legumes collected in the natural resource’s gene bank of Iran. Research Institute of Forests and Rangelands, Tehran, Iran, 103pp.
Huziwara, Y. 1962: Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosomes of Aster. -Am. J. Bot. 49: 116-119.
Javadi, H., Salehi Shanijani, P. & Safavi, S.R. 2019: Chromosome counts and karyomorphology of some species of Astragalus (Fabaceae) from Iran. -Chromosome Science. 22 (1-4): 3-12.
Levan, A., Fredgra, K., Sandberg, A. A. 1964: Nomenclature for centromeric position on chromosomes.  -Hereditas. 52: 201-220.
Maassoumi, A. A. 1987: Notes on the genus Astragalus in Iran I, cytotaxonomic studies on some species. -Iran J. Bot. 3 (2): 117-128.
Maassoumi, A. A. 1989: Notes on the genus Astragalus in Iran IV, cytotaxonomic studies on some species. -Iran J. Bot. 4 (2): 153-163.
Maassoumi, A. A. 2003: Flora of Iran No.43, Astragalus I. Tehran: Research Institute of Forests and Rangeland. 384 pp.
Maassoumi, A. A. 2016a: Flora of Iran No. 85, Astragalus II. Tehran: Research Institute of Forests and Rangeland. 504 pp.
Maassoumi, A. A. 2016b: The role of Astragalus in ecosystem equilibrium, Iran Nature, 1 (1): 47-41.
Magulaev, A. J. 1980: Chromosome numbers of some Fabaceae in North Caucasus. -Botany Zurnal 65: 836-843.
Magulaev, A. J. 1989: Chromosome numbers in some Astragalus (Fabaceae) species of the Caucasus flora. Botaničeskij Žurnal (Moscow & Leningrad). 74: 1519-1521.
Martin, E., Icyer Dogan, G., Karaman Erkul, S. & Eroglu, H. E. 2019: Karyotype analyses of 25 Turkish taxa of Astragalus from the sections Macrophyllum, Hymenostegis, Hymenocoleus, and Anthylloidei (Fabaceae). -Turk. J. Bot. 43: 232-242.
Parfitt, B. D., Baker, M. A. & Gallagher, M. L. 1985: Chromosome Number Reports LXXXVI -Taxon 34: 159-164.
Rani, S., Kumari, S., Gupta, R. C. & Chahota, R. K. 2014: Cytological studies of Angiosperms (174 species) from District Kangra, Himachal Pradesh (India). -Plant Syst. Evol. 300: 851-862.
Ranjbar, M., Karamian, R. & Nouri, S. 2011: Diploid-tetraploid mixoploidy in a new species of Astragalus (Fabaceae) from Iran. -Ann. Bot. Fennici. 48: 343-351.
Ranjbar, M. & Mahmoudian, B. 2015: An overview on cytogenetics of the genus Astragalus subgenus Hypoglottis (Fabaceae). -Caryologia 68 (2): 109-124.
Reeves, A., Tear, J. 2000: Micro measure software. Colorado State University, from http://www. Biology/MicroMeasure
Romero-Zarco, C. 1986: A new method for estimating karyotype asymmetry. -Taxon. 35 (3): 526-530.
Sheidai, M., Maassoumi, A. A. & Pakravan, M. 1996: Karyotypes of some Astragalus taxa (sect. Xiphidium BGE) from Iran. -The nucleus 39: 111-113
Sheidai, M. & Gharemaninejad, F. 2008: New chromosome number and karyotype analysis in four Astragalus L. (Fabaceae) species. -Iran. J. Bot. 15 (1): 21-26.
Sheidai, M., Zarre, S., & Ismaeilzadeh, J. 2009: New chromosome number reports in tragacanthic Astragalus species. -Caryologia 62 (1): 30-36.
Spellenberg, R. 1976: Chromosome numbers and their cytotaxonomic significance for North American Astragalus (Fabaceae). -Taxon 25: 463-476.
Stebbins, G.L. 1971: Chromosomal Evolution in Higher Plants; Edward Arnold: London, 216p.
Yousefzadeh, K., Houshmand, S. & Zamani Dadane, G. 2010: Karyotype analysis of Astragalus effusus Bunge (Fabaceae). -Caryologia 63: 257-261.
Zarre, S., & Azani, N. 2013: Perspectives in taxonomy and phylogeny of the genus Astragalus (Fabaceae): a review. Progress in Biological Sciences, 3, 16.
Zhukova, P. G. 1983: Chromosome numbers of some species of the family Fabaceae from North-East Asia. -Botaniceskjij Žurnal (Moscow & Leningrad) 68 (7): 925-932.