TAXONOMIC SIGNIFICANCE OF POLLEN AND SEED MICROMORPHOLOGY IN THE GENUS HESPERIS L. (BRASSICACEAE)

Document Type : Research Paper

Authors

1 Department of Biology, Faculty of Science, Lorestan University

2 Department of Botany, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), P. O. Box 13185-116, Tehran, Iran.

Abstract

Genus Hesperis L. belongs to Brassicaceae (Cruciferae) family including 46 species in the world, of which seven taxa are distributed in Iran. Pollen and seed macro- and micromorphological features of the Iranian taxa belonging to three sections, including sects. Hesperis, Diaplictos and Pachycarpos were examined for the first time using light (LM) and scanning electron microscope (SEM). Data analyses were performed using PAST (ver. 3.14) software. The results showed that in Hesperis pollen grains are radially symmetrical, monad, isopolar, tricolpate and have a small size. Pollen grain shape is elliptic to circular in equatorial view and circular in polar view. The exine ornamentation is reticulate in all studied specimens. Seed sculpturing pattern revealed two main groups based on the presence or absence of papillae. This study clarified that both pollen and seed macro- and micromorphological characters are significant features in taxonomic classification of the genus Hesperis. Unweighted Pair Group Method with arithmetic mean (UPGMA) and Principle Component Analysis (PCA), strongly supported the sectional delimitation of taxa for most characters but not for seed qualitative characters. The results are mostly in agreement with the previous studies.

Keywords

Article Title [Persian]

اهمیّت تاکسونومیک ریزریخت‌شناسی دانه گرده و بذر سرده Hesperis L. (تیره شب بوئیان)

Authors [Persian]

  • آتنا اسلامی فاروجی 1
  • حامد خدایاری 1
  • مصطفی اسدی 2
1 دانشجوی دکتری سیستماتیک گیاهی گروه زیست‌شناسی دانشگاه لرستان
2 استاد مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
Abstract [Persian]

سرده Hesperis L. متعلّق به تیره شب­بوئیان (Cruciferae) است و شامل 46 گونه در جهان می­باشد، از این میان هفت تاکسون آن در ایران پراکنش دارد. صفات ریخت­شناسی و ریزریخت­ شناسی دانه گرده و دانه تاکسون­های ایرانی Hesperis متعلّق به سه بخش Hesperis، Diaplictos و Pachycarpos با استفاده از میکروسکوپ نوری (LM) و میکروسکوپ الکترونی نگاره (SEM) برای اوّلین بار مطالعه شد. تحلیل داده­ها با استفاده از نرم­افزار PAST (نسخه 14/3) انجام شد. نتایج نشان داد که دانه­های گرده Hesperis متقارن شعاعی، موناد، جورقطب، سه شیاری و دارای اندازه کوچک هستند. شکل دانه گرده در نمای استوایی بیضوی تا مدور و در نمای قطبی مدور است. تزئینات اگزین در تمام نمونه­های مورد مطالعه مشبک است. الگوی تزئینات دانه دو گروه اصلی را بر اساس حضور و عدم حضور زگیل آشکار نمود. این مطالعه روشن نمود که صفات ریختی و ریزریخت­شناسی دانه گرده و دانه صفات مهمّی در طبقه ­بندی تاکسونومیک سرده Hesperis هستند زیرا تحلیل جفت گروه بدون وزن با میانگین حسابی (UPGMA) و تجزیه مؤلفه اصلی (PCA) به جز در صفات کیفی دانه به شدّت از تعیین حدود بخش­ها حمایت می­کند. نتایج عمدتاً با مطالعات پیشین انجام­ شده مطابقت دارد.

Keywords [Persian]

  • Brassicaceae
  • Hesperis
  • microsculpturing
  • pollen
  • seed
  • Iran

References

Abdel khalik, K., Maesen, L. J. G. Van Der, Koopma, N. W. J. M. & Van Denberg, R.G. 2002: Numerical taxonomic study of some tribes of Brassicaceae from Egypt. –Plant Syst. Evol. 233: 207–221.
Al-Shehbaz, I. A. 1989: Systematic and phylogeny of Schizopetalon (Brassicaceae). –Harvard Pap. Bot. 1: 10–46.
Al-Shehbaz, I. A., Beilstein, M. A. & Kellogg, E. A. 2006: Systematics and phylogeny of the Brassicaceae (Cruciferae): An overview. –Pl. Syst. Evol. 259: 89–120.
Al-Shehbaz, I. A. 2012: A generic and tribal synopsis of the Brassicaceae (Cruciferae). –Taxon 61: 931–954.
Andrzejewski, A. L. 1821: Hesperis L. –DC. Syst 2. pp. 447-448.
Aras, S., Duran, A., Yenilmez, G. & Duman, D.C. 2009: Genetic relationships among some Hesperis L. (Brassicaceae) species from Turkey assessed by RAPD analysis. –Afr. Journ. Biot.8: 3128-3134.
Assadi, M., Sajedi, S., Fakhr Ranjbari, H., Mirzadeh Vaghefi, S., Moazzeni, H., Khodashenas, M., Khosravi, A.R., Hatami, A., Mehrnia, M., Kaffash, Sh., Heidarnia, N., Sheidai, M., Heidari Rikan, M., Kavousi, K., Sonboli, A., Veiskarami, Gh. & Aminian, F. 2017: Hesperis L. In: Assadi, M. & Maassoumi, A. A. (Eds.) Flora of Iran. Vol. 143. Research Institute of Forests and Rangelands, Tehran, pp. 647-663. [In Persian]
Barthlott, W. 1981: Epidermal and seed surface characters of plants: systematic applicability and some evolutionary aspects. –Nord. J. Bot. 1: 345–355.
Barthlott, W. 1984: Microstructural features and seed surface features. In: Heywood, V., Moore, D.M. (Eds.), Current Concepts in Plant Taxonomy. –Academic Press, London, pp. 95–105.
Bolurian, S. 2009: A systematic study of certain species of the Alyssum belonging to the Mustard family (Brassicaceae) in Iran. M.A. –thesis, AL-Zahra Univ., Tehran
Bona, M. 2013: Seed-coat microsculpturing of Turkish Lepidium (Brassicaceae) and its systematic application. –Turk. J. Bot. 37: 662–668.
Bouman, F. 1975: Integument initiation and testa development in some Cruciferae. –Bot. J. Linn. Soc. 70, 213–299.
Brochmann, C. 1992: Pollen and seed morphology of Nordic Draba (Brassicaceae): phylogenetic and ecological implications. –Nord J Bot1. pp. 657-673.
Buth, G. M. & Roshan Ara, N.A. 1987: Seed and seed coat anatomy of the some members of tribe Arabideae (Brassicaceae). –Phytomorphology. 37: 341–348.
Doğan, C. & İnceoğlu, O. 1990: Pollen morphology of some Isatis L. Taxa in Turkey. –Turk J Bot. 14: 12-31.
Duran, A., Ünal, F. & Pınar, M. 2003: The Revision of the Genus Hesperis L. in Turkey. The Scientific and Technical Research Council of Turkey, Ankara, XV + 293 pp.
Duran, A. & Ocak, A. 2005: Hesperis turkmendaghensis (Sect. Hesperis) (Cruciferae/Brassicaceae), a new species from the Central Anatolia region, Turkey. –Bot. J. Lin. Soc. 147: 239-247.
Duran, A. 2008: Two new species with pendulous fruits in Hesperis (Brassicaceae) from South Anatolia region, Turkey. –Novon. 18: 453-463.
Duran, A. 2009: Hesperis ozcelikii (Brassicaceae), a new species from Turkey. –Ann. Bot. Fen.46: 577-584.
Duran, A., Ünal, F., Pınar, N.M. & Çetin, Ö. 2011: Morphological, palynological, seed-coat surface and karyological studies of Hesperis bicuspidata and H. stellata (Cruciferae) from Turkey. –Nordic J. Bot.29: 641-651.
Duran, A. 2016a: A taxonomic update of the genus Hesperis in Turkey. 5th International Conference on Agriculture, Environment and Biological Sciences (ICAEBS-16), Pattaya, Thailand, pp. 26-29.
Dvořák, F. 1965:A Note on two species of the genus Hesperis L. in the Near East.. –Fed. Repert. 72: 19-24.
Dvořák, F. 1966: Hesperis luristanica Dvořáksp. nov. –Ann. des Natu. Mus. 69: 29-33.
Dvořák, F. 1968: Hesperis L. In: Rechinger K.H. (Eds.) Flora Iranica. Vol. 57/28.2. AkademischeDruck-u, Verlagsanstalt, –Graz. pp. 266-273.
Dvořák, F. 1973: Infrageneric classification of Hesperis L. –Feddes. Rep. 84: 259-272.
Esfandani-Bozchaloyi, S. & Keshavarzi, M. 2014: Micro- and macromorphological study of Stellaria (Caryophyllaceae) and its closest relatives in Iran. –Phyto. Balc. 20 (2): 179 – 197.
Erdtman, G. 1952: Pollen morphology and plant taxonomy.Angiosperms.Almqvist and Wiksells. - Stockholm.
Eslami Farouji, A., Khodayari, H., Assadi, M., Özüdoğru, B., Çetin, Ö., Mummenhoff, K. & Bhattacharya, S. 2018: Numerical taxonomy contributes to delimitation of Iranian and Turkish Hesperis L. (Brassicaceae) species. Phytotaxa [Unpublished].
Faegri, K. & Iversen, J. 1975: Textbook of pollen analysis. –Hafner Press, New York.
Franzke, A., Lysak, M. A., Al-Shehbaz, I. A., Koch, M. A. & Mummenhoff, K. 2011: Cabbage family affairs: the evolutionary history of Brassicaceae. –Trends Plant Sci. 16: 108–116.
Gower, J.C. 1971: A general coefficient of similarity and some of its properties.–Biometrics. 27: 857-871.
Fournier, M. E. 1868: Monographie du genre Hesperis. –Bul. de la Soc. Bot.de Fra. 13: 326-362.
Hammer, Ø. & Harper, D.A.T. 2006: Paleontological Data Analysis. –Blackwell Publishing, Oxford, 351 pp.
Harley, M. M. 1992: The potential value of pollen morphology as an additional taxonomic character in subtribe Ociminae (Ocimeae, Nepetoideae, Labiatae). In: Harley RM, Reynolds T, editors. Advances in Labiatae Science. 125–138. Richmond, UK: Royal Botanic Gardens, Kew. 125–138.
Huang, C. H., Sun, R., Zeng, Y. H., Zhang, N., Cai, L., Zhang, Q., Koch, M. A., Al-Shehbaz, I. A., Edger, P. P., Pires, J. C., Tan, D. Y., Zhong, Y., Ma, H., 2016: Resolution of Brassicaceae phylogeny using nuclear genes uncovers nested radiations and supports convergent morphological evolution. –Molec. Biol. Evol. 33: 394–412
İnceoğlu, O. & Karamustafa, F. 1977: The pollen morphology of plants in Ankara region II. –Cruciferae. Communications. 21: 111-118.
Keshavarzi, M., Abbasian, S. & Sheidai, M. 2012: Pollen morphology of the genus Clypeola (Brassicaceae) in Iran. –Phyto. Balc. 18 (1): 17-24.
Keshavarzi, M. & Esfandani –Bozchaloyi, S. 2014a: Leaf and Stem Comparative Anatomical Analysis of Three Genera of Alsinoideae (Caryophyllaceae). –Iran. J. Bot. 20 (1): 71-79.
Keshavarzi, M. & Esfandani–Bozchaloyi, S. 2014b: Chromosome Numbers for Some Stellaria L. (Caryophyllaceae) Species and Related Taxa in Iran. –Iran. J. Bot. 20 (1): 36-40.
Koch, M. & Mummenhoff, K. 2001: Thlaspi s.str. (Brassicaceae) versus Thlaspi s. l.: morphological and anatomical characters in the light of ITS nrDNA sequence data. –Pl. Syst. Evol. 227: 209–225.
Koul, K. K., Nagpal, R. & Raina, S. N., 2000: Seed coat microsculpturing in Brassica and allied genera (subtribe Brassicinae, Raphaninae, Moricandiinae). –Ann. Bot. 86: 385–397.
Meyer, F. K. 1973: Conspectus der Thlaspi-arten Europas: afrikas und vorderasiens. –Feddes Rep. 84: 449–469.
Meyer, F. K. 1979: Kritische revision der Thlaspi-arten Europas: afrikas und vorderasiens. –Feddes Rep. 90: 129–154.
Meyer, F. K. 1991: Seed-coat anatomy as a character for a new classification of Thlaspi. –Flora Veg. Mundi 9: 9–15.
Mummenhoff, K. & Koch, M. 1994: Chloroplast DNA restriction site variation and phylogenetic relationships in the genus Thlaspi sensu lato (Brassicaceae). –Syst. Bot. 19: 73–88.
Mummenhoff, K., Franzke, A. & Koch, M. 1997a: Molecular data reveal convergence in fruit characters used in the classification of Thlaspi s. l. (Brassicaceae). –Bot. J. Linn. Soc. 125: 183–199.
Mummenhoff, K., Franzke, A. & Koch, M. 1997b: Molecular phylogenetics of Thlaspi s. l. (Brassicaceae) based on chloroplast DNA restriction site variation and sequences of the internal transcribed spacers of nuclear ribosomal DNA. –Canad. J. Bot. 75: 469–482.
Murley, M. R. 1951: Seeds of the Cruciferae of North Eastern America. –Am Midl Nat. 46: 1-81.
Mutlu, B. and Erik, S. 2012: Pollen morphology and its taxonomic significance of the genus Arabis (Brassicaceae) in Turkey. –Plant Syst. Evol. 298: 1931–1946.
Özüdoğru, B., Galip, A., Erik, S. & Mummenoff, K. 2016: Seed morphology of Ricotia (Brassicaceae) and its phylogenetic and systematic implication. –Elsevier, Flora. 222: 60-67.
Padure, I.M., Zernig, K., Heber, G. & Simic, S. 2016: Morphological aspects and SEM studies of pollen and seed in
Hesperis matronalis subsp. matronalis and H. matronalis subsp. candida (Brassicaceae) from Austria. 17th Treffen der Österreichischen Botanikerinnen und Botaniker. p. 82.
Perveen, A., Qaiser, M. & Khan, R.2004. Pollen Flora of Pakistan – XLII. Brassicaceae. –Pakistan J. Bot. 36: 683-700.
Pinar, N. M., Duran, A., Ceter, T. & Tug, G. N. 2009: Pollen and seed morphology of the genus Hesperis L. (Brassicaceae) in Turkey. –Turk J. Bot, 33: 83-96.
Punt, W., Hoen, P. O., Blackmore, S., Nilsson, S. and Thomas, A. L. 2007: Glossary of pollen terminology. -Rev. Palaeobot. Palynol. 143: 1–81.
Rollins, R. C. 1979: Dithyrea and a related genus (Cruciferae). –Bussey Inst. Harvard Univ. 1979: 3–20.
Tekin, M. & Martin, E. 2017: Morphology, anatomy, and karyology of endangered Turkish endemic Physoptychis haussknechtii Bornm. (Brassicaceae) from Central Anatolia. Acta Bot. Croat. 76 (1): 32-40.
Vaughan, J. G. & Whitehouse, J. M. 1971: Seed structure and the taxonomy of Cruciferae. –Bot. J. Linn. Soc. 64: 383–409.
Zunk, K., Mummenhoff, K., Koch, M. & Hurka, H. 1996: Phylogenetic relationships of Thlaspi s. l. (subtribe Thlaspidinae, Lepidieae) and allied genera based on chloroplast DNA restriction-site variation. –Theor. Appl. Genet. 92: 375–381.
The Iranian Journal of Botany
Volume 24, Issue 2
December 2018
Pages 91-105

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History

  • Receive Date: 11 March 2018
  • Revise Date: 16 September 2018
  • Accept Date: 17 October 2018

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