PHYLOGENETIC ANALYSIS AMONG FOUR SECTIONS OF GENUS DENDROBIUM SW. (ORCHIDACEAE) BASED ON LOW COPY NUCLEAR GENE (XDH) SEQUENCES IN PENINSULAR MALAYSIA

Document Type : Research Paper

Authors

1 Faculty of Science, University of Birjand, Birjand, South Khorasan, Iran.

2 Department of Biology, Faculty of Science, University Putra Malaysia & Institute of Tropical Forestry & Forest Products, University Putra Malaysia

Abstract

The present study focused on the molecular phylogeny of the four sections (Aporum, Crumenata, Strongyle, and Bolbidium) of genus Dendrobium SW. (Orchidaceae) based upon low copy nuclear gene, Xanthine Dehydrogenase (Xdh) sequences using maximum parsimony (MP), maximum likelihood (ML) and Bayesian (BI) methods. Single and low copy nuclear genes have been increasingly used in phylogenetic reconstruction because they contain large amounts of genetic information and are biparentally inherited. Classifications based on morphological characters have not been able to clearly divide these four sections and neither do they support their monophyletic origins. Therefore, detailed analysis using molecular data is required to ascertain their status. This study includes 20 species of genus Dendrobium and 2 species from genus Bulbophyllum (section Sestochilus) as outgroup taxa. According to the results, the aligned sequences consisted of 733 nucleotide characters of which 131characters were parsimony informative.  The analysis revealed that the nuclear genes can be reliable marker for the phylogenetic study of genus Dendrobium. The results suggested that the four sections are probably best considered as one section instead of four and based on the International code of Botanical Nomenclature (ICBN) rules can be named as section Aporum for this new classification.

Keywords

Article Title [Persian]

فیلوژنی چهار بخشه از جنس .Orchidaceae) Dendrobium SW) بر اساس ترکیب داده های توالی ژن هسته ایXdh در شبه جزیره مالزی

Authors [Persian]

  • مریم مودی 1
  • روسه آ گو 2
1 دانشکده علوم دانشگاه بیرجند، خراسان جنوبی
2 دپارتمان بیولوژی، دانشکده علوم و دانشکده جنگلداری گرمسیری و محصولات جنگلی، دانشگاه پوتورا ، مالزی
Abstract [Persian]

در مطالعه حاضر با استفاده ازتوالی ژن هسته ای Xdh بازسازی فیلوژنی چهار بخشه از جنس Dendrobium متعلق به تیره گیاهی Orchidaceae با روشهای بیشینه صرفه جویی، درست نمایی حداکثر و بیسین صورت گرفته است. ژن های هسته ای با نسخه کم برای مطالعات فیلوژنتیکی مورد استفاده قرار می گیرند. این ژن ها دارای مقادیر زیادی از اطلاعات ژنتیکی و توارثی از هر دو والد می باشند. مطالعات مورفولوژیکی در ارتباط با مونوفیلی چهار بخشه (Aporum, Crumenata, Strongyle and Bolbidium) قابل استناد نمی باشد. لذا برای دستیابی به اطلاعات دقیقتر نیاز به مطالعات مولکولی می باشد. در مطالعه حاضر 20 گونه گیاهی متعلق به جنس Dendrobium به همراه دو گونه گیاهی از جنس ( Bulbophyllum   (section Sestochilus به عنوان برون گروه مورد بررسی قرار گرفته است.  بر پایه نتایج ، توالی نوکلئوتیدی همردیف سازی شده دارای 733 جایگاه نوکلئوتیدی می باشد که از این تعداد 133جفت باز به لحاظ پارسیمونی اطلاعاتی است. آنالیز داده ها نشان می دهد که ژن های هسته ای می توانند به عنوان یک نشانگر معتبر مولکولی برای مطالعات فیلوژنتیکی خصوصا در ارتباط با این جنس گیاهی مورد استفاده قرار گیرند. با توجه به نتایج به دست آمده ، پیشنهاد می شود که بهتر است چهار بخشه به صورت یک بخشه در نظر گرفته شده و بر اساس قوانین بین المللی نامگذاری گیاهان به نام بخشه Aporum نامیده شود.

Keywords [Persian]

  • Dendrobium
  • Orchidaceae
  • low copy nuclear gene
  • Xdh
  • monophyly
  • phylogeney

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The Iranian Journal of Botany
Volume 21, Issue 2 - Serial Number 2
December 2015
Pages 169-178

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History

  • Receive Date: 06 September 2015
  • Revise Date: 18 November 2015
  • Accept Date: 12 December 2015

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