KARYOTYPE VARIATION AND GENOMIC CHARACTERIZATION IN FIVE MONOCOTYLEDONOUS MANGROVE ASSOCIATE FROM ORISSA COAST

Document Type : Research Paper

Authors

Cytogenetics Laboratory, Regional Plant Resource Centre, Bhubaneswar 751015, Orissa, India

Abstract

Chromosome number and karyotype analysis of five monocot mangrove associates from Dhamra and Paradeep coast of Orissa revealed 2n=48 in Porteresia coarctata; 2n=22 in Crinum defixum and Cryptocoryne ciliata and 2n=20 in Asparagus racemosus var. javanica and Cyperus cephalotes. Total chromosome length varied from 42.44 µm in A. racemosus var. javanica to 93.86 µm in C. defixum. Significant variation of chromosome volumes were also recorded among the species. The 4C DNA content varied significantly from 47.15 pg in C. defixum to 6.31pg in C. cephalotes. A positive significant correlation between the chromosome volume and 4C nuclear DNA content among five different species was revealed. The genome size was around 1,545 mbp in C. cephalotes to 4,074 mbp in P. coarctata with a highest size (11,551 mbp) in C. defixum that suggests the high repetitive DNA sequences in the Crinum with highest chromosome length and volume of this species.
 

Keywords

Article Title [Persian]

گوناگونی کاریوتیپ و ویژگی ژنوم در پنج مانگرو تک‌لپه از ساحل اوریسا، هند

Authors [Persian]

  • اس ینا
  • آنات باندهو داس
آزمایشگاه سیتوژنتیک مرکز تحقیقات گیاهی بهوبانسوار اوریسای هند
Abstract [Persian]

شمارش کروموزومی و تجزیه کاریوتیپ تعداد پنج مانگرو تک‌لپه از در دارما و ساحل پارادیپ در اوریسا (هندوستان) نشان داد که در گونه 48=n2   Porteresia coarctata ، در گونه‌های Crinum defixum و 22=n2 Cryptocaryne ciliata ، در گونه‌های Asparagus racemosus var. javanica و Cyperus cephalotes 20=n2  می‌باشد. طول کل کروموزومی از 44/42 در گونه Asparagus racemosus var. javanica تا 86/93 در گونه Crinum defixum تغییر می‌کند. حجم کروموزومی در گونه‌های مختلف تغییرات زیادی را نشان می‌دهد. محتوای 4C DNA  از pg 15/47 در گونه Crinum defixum تا  pg 31/6 در گونه Cyperus cephalotes تغییر می‌یابد. همبستگی معنی‌دار مثبتی بین حجم کروموزومی و محتوی DNA 4C هسته در بین پنج گونه مشاهده گردید. اندازه ژنوم در حدود mbp 1545 در گونه Cyperus cephalotes تا mbp 4074 در گونه Porteresia coarctata  است و حداکثر آن mbp 11551 در گونه Crinum defixum می‌باشد. این موضوع بیانگر توالی DNA بالا با بیشترین طول و حجم کروموزومی در جنس Crinum است.

Keywords [Persian]

  • genome size
  • Karyotype
  • somatic chromosome
  • Crinum
  • Cryptocoryne
  • Asparagus
  • Cyperus
  • Porteresia

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History

  • Receive Date: 15 December 2015
  • Accept Date: 15 December 2015

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