CYTOPHOTOMETRIC ESTIMATION OF 4C DNA AND KARYOTYPE ANALYSIS IN TEN CULTIVARS OF TRIGONELLA FOENUM-GRAECUM - II.

Document Type : Research Paper

Authors

Cytogenetics Laboratory, Regional Plant Resource Centre, Bhubaneswar 751015, Orissa, India.

Abstract

 
Cytophotodensitometric estimation of 4C DNA content in root tip cells and karyotype analysis were carried out in ten cultivars of fenugreek (Trigonella foenum-graecum). Somatic chromosome number was 2n=16 in all the cultivars. Detailed karyotype analysis revealed cultivar specific chromosomal characteristics and minute structural alterations in chromosomes of the genome. The 4C DNA content varied significantly from 9.116pg in the cultivar TG-41 to 12.420 pg in TG-03. Significant variations in the chromosome length and volume and total form percentage (TF%) were noted at cultivar level. Correlation coefficient studies revealed interdependence between the chromosome volume and nuclear DNA content of the varieties. Variations in chromosome structure and the nuclear DNA content despite the same somatic chromosome number suggest micro-evolution leading to the development of new cultivars.

Keywords

Article Title [Persian]

تخمین سیتوفتومتری 4C DNA و تجزیه کاریوتیپ ده رقم شنبلیله II

Authors [Persian]

  • آنات باندهو داس
  • سوپراوا موهانتی
  • پرماندانا داس
آزمایشگاه سیتوژنتیک مرکز تحقیقات گیاهی بوبانسوار اوراسیای هند
Abstract [Persian]

تخمین سیتوفتومتری 4C DNA سلولها نوک ریشه و تجزیه کاریوتیپ ده رقم شنبلیله(Trigonella foenum-graecum)  انجام شده است. تعداد کروموزومهای سوماتیک 16=n2 برای همه رقمها ذکر می‌شود. تجزیه جزیی کاریوتیپ نشان دهنده ویژگیهای کروموزومی اختصاصی ارقام و جابجایی ساختاری کمی در کروموزومهای ژنوم است. محتوای 4C DNA  از pg  11/9 در رقم TG-41 تا pg420/12 در رقم TG-03 تغییر نمود. دگرگونی معنی‌داری در طول، حجم و درصد شکل کلی (TF%) در سطح رقم دیده شد. مطالعات ضریب همبستگی نشان دهنده ارتباط بین حجم کروموزوم و محتوای DNA هسته در واریته‌ها است. دگرگونی ساختار کروموزوم و محتوای DNA علیرغم تعداد کروموزومهای برابر نشان دهنده وجود ریز تکامل در جهت توسعه ارقام جدید است.

Keywords [Persian]

  • Fenugreek
  • genome size
  • Karyotype
  • Trigonella

References

Anonymus. 1976: In The Wealth of India. vo1. 10, pp 298-306. Publication and Information Directorate (CSIR)-New Delhi.
Bir S. S. & Kumari S. 1980: Cytological evolution of the leguminous flora of the Punjab plain. In Recent Researches in Plant Sciences. pp 251-260. Edited by S. S. Bir­-Kalyani Publishers. -Ludhiana, India.
Chattopadhyay D. & Sharma A. K. 1990: Chromosome studies and micro spectrophotometric estimation of nuclear DNA in different strains of Coriandrum sativum L. -Cytobios 64: 43-51.
Das A. B. & Mallick R. 1989a: Variation in karyotype and nuclear DNA content in different varieties of Foeniculum vulgare Mill- Cytologia 54:129-34.
Das A. B. & Mallick R. 1989b: Varietal differences in 4C DNA content and chromosome characteristics of Coriandrum sativum L. -Cytologia 54: 609-616.
Das A. 8. & Mallick R. 1993a: Nuclear DNA and chromosomal changes within the tribe Ammineae. -Cytobios 74: 197-207.
Das A. B. & Mallick R. 1993b: Karyotype diversity and interspecific 4C DNA variation in Bupleurum. -Biologia Pl. 35: 335-363.
Das A. B. & Chaterjee A. 1994: Analysis of genome in relation with seed protein diversity of Cassia using polyacrylamide gel-electrophoresis.-Bangladesh Jour. Bot. 23: 167-174.
Das A. B. & Das P. 1994: Estimation of 4C DNA content and karyotype analysis in edible varieties of banana (Musa acuminata). -Cytobios 78:213-220.
Das A. B. & Das P. 1994: Estimation of nuclear DNA and karyotype analysis in nine cultivars of Musa. -Cytobios 90: 181-­192.
Das A. B., Pattanaik M., T. Thungaraj & Das P. 1997: Cytophotometric estimation of nuclear 4C DNA content and karyotype analysis of eight cultivars of Trigonella foenum-graecum. -Cytobios 91: 171-179.
Das A. B., Mohanty, S. & Das, P. 2001. Cytophotometric estimation of 4C DNA content and karyotype analysis in ten cultivars of Trigonella foenum-graecum. -Iran. Journ. Bot. 9 (1): 1-9.
Dhillon S. S. & Miksche J. P. 1982: DNA content and heterochromatin variation in various tissues of peanut (Arachis hypogaea). -Am. J. Bot. 69: 219-226.
Frahm-Leliveld J. A. 1957: Observations cytologiques sur quelques Legumineuses tropicales et subtropicales. -Rev. Cytol. Bioi. Veg. 18: 273-287.
Hammatt N., Blackhall N. W. & Davey M. R. 1991: Variation in the DNA content of Glycine species. -J. exp. Bot. 42: 659-665.
Ignacimuthu S. & Babu C. R. 1988: Nuclear DNA and RNA amounts in the wild and cultivated Urd and Mung beans and their M 1 plants. -Cytologia 53: 535-541.
Lavania U. C. & Sharma A. K. 1980: Giemsa C-banding, somatic association and orientation of interphase chromosomes in Trigonella foenum-graecum (L.). -Caryologia 33: 17-23.
Maxted N., Callimassia M. A. & Bennett M. D. 1991: Cytotaxonomic studies of Eastern Mediterranean Vicia species (Leguminosae). -Plant Syst. Evol. 177: 221-234.
 
Mukharjee S. & Sharma A. K 1986: In situ nuclear DNA quantitation in organs of different strains of Cicer arietinum L. -­Cytobios 48: 151-156.
Ohri D., Kumur A. & Pal M. 1986: Correlations between 2C DNA values and habitat in Cassia (Leguminosae: Caesalpinioideae). -Plant Syst. Evol. 153: 223-227.
Parida A., Raina S. N. & Narayan R. K. J. 1990: Quantitative DNA variation between and within chromosome complements of Vigna species (Fabaceae). -Genetica 82: 125-133.
Price H. J. 1976: Evolution of DNA content in higher plants. -Bot. Rev. 42: 27-52.
Price H. J., Bachman K., Chambers K. L. & Riggs I. 1980: Detection of interspecific variation in nuclear DNA content in Microseris douglassi. -Bot. Gaz. 141: 195-198.
Raghuvanshi S. S. & Singh A. K. 1974a: A possible mutagenic effect of colchicine in Trigonella foenum-graecum. -Caryologia 39: 73-76.
Raghuvanshi S. S. & Singh A. K. 1974b: Studies on the effect of gamma rays on Trigonella foenum-graecum. -Cytologia 39: 473-482.
Raina S. N & Bisht M. S. 1988: DNA amounts and chromatin compactness in Vicia.­ -Genetica 77: 65-77.
Sharma A. K. & Sharma A. 1980: Chromosome techniques theory and practice. Third edition. -Butterworths, London.
Singh A. & Roy R. P. 1970: Karyotype studies in Trigonella, Indigofera and Phaseolus. -Nucleus 13: 41-54.
Sokal P. R. & Rohlf F. I. 1973: Introduction to Biostatistic. -Freeman, San Franscisco.
Van't hof J. 1965: Relationships between mitotic cycle duration, S period duration and the average rate of DNA synthesis in the root meristem cells of several plants.­ -Expl Cell Res. 39: 48.
Yamaguchi Y. & Tsunoda S. 1969. Nuclear volume, nuclear DNA content and radiosensitivity in Brassica and allied genera. -Jap. J. Breed. 19: 350-356.
The Iranian Journal of Botany
Volume 9, Issue 2 - Serial Number 2
December 2002
Pages 151-159

Files

History

  • Receive Date: 14 December 2015
  • Accept Date: 14 December 2015

Share

How to cite

Statistics