MOLECULAR PHYLOGENY OF THE TRIBE HEDYSAREAE WITH SPECIAL REFERENCE TO ONOBRYCHIS (FABACEAE) AS INFERRED FROM nrDNA ITS SEQUENCES

Document Type : Research Paper

Authors

1 Department of Plant Biology, Faculty of Basic Sciences, Tarbiat Modares University, Tehran 14115-175, Iran

2 Research Institute of Forests and Rangelands, P. O. Box 13185-116, Tehran, Iran

Abstract

A total of 53 species (54 sequence accessions) representing 28 species of the tribe Hedysareae, 5 species of Vicioid clade and 19 species of Galegeae including the two Glycyrrhiza species as outgroups was included in a phylogenetic analysis using nrDNA ITS sequences with maximum parsimony method. The analysis, based on successive reweighting by rescaled consistency index, revealed that Vicioid clade is the first branch followed by Chesneya-Caragana clade and Astragalean clade as successive sisters to Hedysaroid clade. The genus Alhagi is well allied, as the basal most taxon, with the remainder of Hedysaroid clade. Ebenus and Taverniera are each monophyletic and weakly allied taxa. Hedysarum is not monophyletic, its species are dispersed across Hedysaroid clade. Sulla a segregate of Hedysarum, forms a well supported monophyletic group with the inclusion of H. humile. The enigmatic genus Eversmannia is allied with Onobrychis clade. Onobrychis itself is not monophyletic due to inclusion of the two Hedysarum species, and Onobrychis subgenus Onobrychis is not monophyletic as well, whereas Onobrychis subgenus Sisyrosema forms a strongly supported clade. In contrast to Onobrychis section Heliobrychis, sections Dendrobrychis and Onobrychis appear not to be each monophyletic. Hedysareae is circumscribed herein to include only Alhagi, Ebenus, Eversmannia, Hedysarum, Onobrychis, Sulla, Taverniera, Coretrodendron and Sartoria.

Keywords

Article Title [Persian]

فیلوژنی مولکولی قبیله Hedysareae با تأکید ویژه بر جنس Onobrychis بر اساس توالی‌های nrDNA ITS

Authors [Persian]

  • سمیه آهنگریان 1
  • شاهرخ کاظم پور اوصالو 1
  • علی اصغر معصومی 2
1 گروه زیست گیاهی، دانشکده علوم پایه،دانشگاه تربیت مدرس، تهران-ایران
2 مؤسسه تحقیقات جنگل ها و مراتع، صندوق پستی: 116-13185، تهران-ایران
Abstract [Persian]

تعداد 53 گونه (54 توالی) شامل 28 گونه از قبیله Hedysareae، 5 گونه از کلاد Vicioid و 19 گونه از قبیله Galegeae از جمله دو گونه Glycyrrhiza به عنوان برون‌گروه با استفاده از توالی‌های nrDNA ITS در آنالیز فیلوژنی با روش بیشینه صرفه‌جویی قرار گرفت. آنالیز براساس وزن‌دهی مجدد پی‌درپی با شاخص سازگاری تصحیح شده فاش نمود که کلاد Vicioid اولین شاخه‌ای است که بدنبال آن کلاد Chesneya-Caragana و کلاد Astragalean به عنوان گروه‌های خواهری کلاد Hedysaroid قرار دارند. جنس Alhagi به‌عنوان پایه‌ترین تاکسون با بقیه کلاد Hedysaroid به خوبی متحد می‌شود. هرکدام از جنس‌های Ebenus و Taverniera تک‌تبارند و به طور ضعیف با هم متحد هستند. جنس Hedysarum تک‌تبار نیست و گونه‌هایش در سراسر کلاد Hedysaroid پخش هستند. Sulla یک جنس جداشده از Hedysarum، با در برگرفتن گونه Hedysarum humile گروه تک‌تبار به خوبی حمایت شده‌ای را تشکیل می‌دهد. جنس Eversmanniaبا کلاد Onobrychis خویشاوند است. خود جنس Onobrychis به علت قرار گرفتن دو گونه Hedysarum در میان گونه‌هایش تک‌تبار نیست و زیرجنس Onobrychis از جنس Onobrychis نیز تک‌تبار نیست، در حالیکه زیرجنس Sisyrosema یک کلاد قویاً حمایت شده‌ای را می‌سازد. در میان بخشه‌های چند گونه‌ای آنالیز شده از جنس Onobrychis، برخلاف بخشه Heliobrychis، بخشه‌های Dendrobrychis و Onobrychis ظاهراً تک‌تبار نیستند. در اینجا قبیله Hedysareac فقط مشتمل بر جنس‌های Alhagi, Ebenus, Eversmannia, Hedysarum, Onobrychis, Sulla, Taverniera, Corethrodendron  و Sartoriaتعیین حدود می‌شود.


Keywords [Persian]

  • Fabaceae
  • Hedysareae
  • Molecular Phylogeny
  • nrDNA ITS
  • Onobrychis
  • Iran

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The Iranian Journal of Botany
Volume 13, Issue 2 - Serial Number 2
November 2007
Pages 64-74

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History

  • Receive Date: 15 October 2007
  • Revise Date: 19 December 2015
  • Accept Date: 19 December 2007

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