TEMPORAL GENETIC STRUCTURE OF IRANIAN POPULATIONS OF BEECH, FAGUS ORIENTALIS (FAGACEAE)

Document Type : Research Paper

Authors

1 Research Institute of Forests and Rangelands, P. O. Box 13185-116, Tehran, Iran

2 Institute of Plant Genetic, CNR, Via Madonna del Piano, I-50019 Sesto Fiorentino, Firenze, Italy

Abstract

Reforestation with autochthonous species should take into account the preservation of the temporal variability and the geographic structure of genetic diversity in forest species. In order to provide empirical data about the suitability of methods of sampling material, genetic comparison of 10 beech populations (at least 40 trees per population) and their progenies (seeds of 10 mother trees per population, each tree 7 seeds) were analysed using four highly polymorphic microsatellite loci. The allelic multiplicity was higher in seed samples than adult trees indicating gene flow from adjacent plant populations. The comparison for genetic diversity measures between adult trees and seed generation revealed no significant differences for allelic richness (Na), effective number of alleles (Ne), and number of rare alleles (Nr), neither observed (Ho) nor expected heterozygosity (He). Genetic differentiation in allelic frequencies between adult trees and seeds generation were rather low (Fst = 0.058). A close genetic relationship between adult trees from seed generation of each population, which revealed by un-weighted pair group method based on arithmetic average (UPGMA) and supported by an analysis of molecular variance (AMOVA), were detected.  In this paper some aspects related to seed sampling were discussed.

Keywords

Article Title [Persian]

ساختار ژنتیکی زمانی جمعیت‌هایی از گونه راش (Fagus orientalis) در ایران

Authors [Persian]

  • پروین صالحی شانجانی 1
  • جووانی جوزپه وندرامین 2
  • محسن کلاگری 1
1 استادیار پژوهش،مؤسسه تحقیقات جنگلها و مراتع کشور
2 استاد، مؤسسه تحقیقات ژنتیک گیاهی، CNR ، فلورانس، ایتالیا
Abstract [Persian]

در جنگاکاری با گونه های بومی می بایست حفاظت از گوناگونی زمانی و ساختار جغرافیایی تنوع ژنتیکی گونه‌های جنگلی در نظر گرفته شود. برای تهیه اطلاعات کاربردی در مورد کارآمدی روشهای جمع‌آوری نمونه، ترکیب ژنتیکی ده جمعیت راش (حداقل 40 درخت در هر جمعیت) و نتاج آنها (بذور 10 درخت مادری در هر جمعیت، به میزان 7 بذر از هر درخت) توسط چهار لوکوس میکروساتلایتی پلی‌مورف بررسی شد. تکثّر آللی در نمونه‌های بذر بیش از درختان بالغ بود که حاکی از وجود جریان ژن از جمعیتهای گیاهی مجاور است. مقایسه مقادیر تنوع ژنتیکی بین درختان بالغ و نسل بذری هیچ اختلافی را از نظر غنای آللی (Na)، تعداد موثر آللها (Ne)، تعداد آللهای نادر (Nr)، هتروزیگوزیتی مشاهده شده (Ho) و هتروزیگوزیتی مورد انتظار (He) نشان ندادند. تمایز ژنتیکی در فراوانی آللی بین درختان بالغ و نسل بذور نیز بسیار کم بود (Fst = 058/0). روابط ژنتیکی نزدیکی بین درختان بالغ و نسل بذری در هر جمعیت وجود داشت که بوسیله روش معدل گروهی (UPGMA) نشان داده شد و آنالیز واریانس ملکولی (AMOVA) نیز آن را تأیید نمود. در این پژوهش برخی ویژگیهای جمع‌آوری بذر مورد بحث قرار گرفت.

Keywords [Persian]

  • Fagus orientalis
  • Hyrcanian forests
  • Genetic diversity
  • microsatellite
  • Gene flow
  • Iran

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History

  • Receive Date: 18 November 2009
  • Revise Date: 30 December 2009
  • Accept Date: 03 March 2010

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