NEW CHROMOSOME NUMBER AND KARYOTYPE ANALYSIS IN FOUR ASTRAGALUS L. (FABACEAE) SPECIES

Document Type : Research Paper

Authors

Shahid Beheshti University G. C., Faculty of Biological Sciences, Tehran, Iran.

Abstract

Karyotype studies were performed in four Astragalus species of A. cornu-carpae, A. mucronifolius, A. talimansurensis from the section Leucocersis and A. crysostachys from the section Hymenostegis. All four species showed 2n = 2x = 16 chromosome number and had metacentric and sub-metacentric chromosomes. The size of chromosomes varied from 0.75 mm in A. talimansurensis to 4.00 mm in A. cornu-carpae. The species studied differed significantly in the size of their chromosomes and in karyotype formulae indicating the role of quantitative change in the chromatin (DNA) material as well as chromosomes structural changes in the species diversification. The chromosome numbers of all four species studied are new to science. PCA ordination of the species showed their karyotype distinctness and that such data are of no use in the sectional delimitation in Astragalus.

Keywords

Article Title [Persian]

گزارش جدید کروموزومی و مطا لعه کاریوتیپی چهار گونه گون ( .Astragalus L) در ایران

Authors [Persian]

  • مسعود شیدایی
  • فرخ قهرمانی نژاد
استاد دانشکده علوم زیستی، دانشگاه شهید بهشتی
Abstract [Persian]

خصوصیات کاریوتیپی چهار گونه گون (Astragalus) با نامهای A. cornu-carpae ،    A. mucronifoliusو   A. talimansurensis از بخشه  Leucocersis  و گونه A. crysostachysاز بخشه  Hymenostegis. مطالعه شدند. گونه ها وجود 2n = 2x = 16  را نشان دادند که تمامی گزارش جدید می‌باشند. کروموزومها از نوع متا و ساب متا بودند و اندازه آنها ازmm  75/. در A. talimansurensis تا 4 mm در A. cornu-carpaeمتغیر بود. گونه‌های مطالعه شده اختلاف معنی داری را در اندازه کروموزومها نشان دادند که بیانگر تغییرات کمی در ماده کروموزومی (DNA) گونه‌ها در فرایند گونه‌زایی می‌باشد. تفاوت در فرمول کاریوتیپی این گونه‌ها وجود داشت که نشاندهنده وقوع تغییرات ساختمانی کروموزومها است. گروه‌بندی گونه‌ها با استفاده از تجزیه به مولفه‌های اصلی (PCA) تمایز کاریوتیپی گونه‌ها را نشان داد و اینکه داده‌های کروموزومی برای تفکیک بخشه‌های گون مفید نمی‌باشند.

Keywords [Persian]

  • Key words. Astragalus
  • Fabaceae
  • Karyotype
  • Iran

References

Aryavand, A. 1977: IOPB chromosome number reports LVII. -Taxon 26: 443-452.
Badr, A., Hamoud, M.A. and El-Rabey, H.A. 1996: Chromosomal studies in the Egyptian flora V. Chromosomal relationships in the genus Astragalus L. (Fabaceae). -Cytologia 60: 105-111
Cartier, D. 1979: Premieres prospections caryologiques du genre Astragalus L. dans la flore du basin mediterraneen oriental. -Revue de Cytologie et de Biologie Végétales, le Botaniste 2: 169-181.
Hu, J.M., Sanderson, M. J. and Wojciechowski, M.F. 2008: Website for the largest genus of vascular plants, Astragalus. http://loco.biosci.arizona.edu/astragalus/astragalus home.htm
Javadi, H. 2006: Study of kryotype in three Astragalus species. -Pajouhesh & Sazandegi 73: 131-135.
Levan, A., Fredga, K. and Sandberg, A. 1964: Nomenclature for centromeric position on chromosomes. -Hereditas 52, 201–220.
Liston, A. 1992a: Variation in the chloroplast genes rpoC1 and rpoC2 of the genus Astragalus (Fabaceae): evidence from restriction site mapping of a PCR-amplified fragment. -American Journal of Boany 79: 953-961.
Liston, A. 1992b: Isozyme systematics of Astragalus sect. Leptocarpi subsect. Californici (Fabaceae). -Systematic Botany 17: 367-379.
Löve, Á. and Löve, D. 1982: IOPB chromosome number reports LXXV. -Taxon 31 (2): 344-360.
Maassoumi, A.A. 1986: Astragalus L., Vol. 1, Annuals Publication, Technical Publication 47. -Research Institute of Forests and Rangelands, Tehran.
Maassoumi, A.A. 1987: Notes on the genus Astragalus in Iran I, cytotaxonomic studies on some species. -Iranian Journal of Botany 3: 117-128.
Mabberley, D.J. 1997: The Plant-Book, A Portable Dictionary of the Vascular Plants, 2nd edition. -Cambridge University Press.
Magulaev, A.J. 1980: Chromosome numbers of some Fabaceae in North Caucasus. - Botany Zurnal 65: 836-843.
Magulaev, A.J. 1989: Chromosome numbers in some Astragalus (Fabaceae) species of the Caucasus flora. -Botaničeskij Žurnal (Moscow & Leningrad). 74: 1519--1521
Parfitt, B.D., Baker, M.A. and Gallagher, M.L. 1985: Chromosome Number Reports LXXXVI.  -Taxon, 34: 159-164 .
Polhill, R.M. 1981: Carmichaelieae. In: Polhill, R. M. and Raven, P.H. (eds.), Advances in Legume Systematics, part 1, 364-366. -Royal Botanical Gardens, Kew, U. K.
Sanderson, M.J. and Doyle, J.J. 1993: Phylogenetic relationships in North American Astragalus (Fabaceae) based on chloroplast DNA restriction site variation. -Systematic Botany 18: 395-408.
Sheidai, M., Maasoumii, A.R. and Pakravan, M. 1996: Karyotypes of some Astragalus taxa (Sec. Xiphidium Bge) from Iran. -Nucleus 39 (3): 111-113.
Sheidai, M. and Rashid, S., 2007: Cytogenetic study of some Hordeum L. species in Iran. -Acta Biol. Zseged. 51: 107-112.
Sheidai, M. and Jalilian, N. 2008: Karyotypic studies in some species and populations of the genus Lotus L. in Iran. -Acta Botanica Croatica 67: 45-52.
Spellenberg, R. 1976: Chromosome numbers and their cytotaxonomic significance for North American Astragalus (Fabaceae). -Taxon 25: 463 - 476.
Stebbins, G. L. 1971: Chromosomal evolution in higher plants. -Edward Arnold, London.
Wojciechowski, M.F., Sanderson, M.J., Baldwin, B.G. and Donoghue, M.J. 1993: Monophyly of anueploid Astragalus (Fabaceae): Evidence from nuclear ribosomal DNA-ITS sequences. -American Journal of Botany 80:711-722.
Wojciechowski, M.F., Sanderson, M.J. and Hu, J.M. 1999: Evidence on the monophyly of Astragalus (Fabaceae) and its major subgroups based on nuclear ribosomal DNA ITS and chloroplast DNA trnL intron data. -Systematic Botany 24: 409-437.
Zarre, M.S. 1999: Systematic revision of Astragalus sect. Adiaspastus, sect. Macrophyllium and sect. Pterophorus (Fabaceae). -Englera 18: 1-219.
Zhukova, P.G. 1983: Chromosome numbers of some species of the family Fabaceae from North-East Asia. -Botaniceskjij Žurnal (Moscow & Leningrad) 68 (7): 925-932.
 
 

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History

  • Receive Date: 20 October 2008
  • Revise Date: 10 November 2008
  • Accept Date: 25 November 2008

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