PLOIDY DETERMINATION OF AEGILOPS CYLINDRICA HOST ACCESSIONS OF IRAN BY USING FLOW CYTOMETRY AND CHROMOSOME COUNTING

Document Type : Research Paper

Authors

1 Young Research Club, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Seed and Plant Improvement Institute, National Plant Gene Bank of Iran, Karaj, Iran

3 College of Agriculture and Natural Resources, Tehran University

4 Islamic Azad University, Science and Research Branch, Tehran, Iran

Abstract

Chromosomes counting and flow cytometry method were used for assessing the ploidy level of various Aegilops cylindrica Host (2n=4x=28, CCDD) accessions of the National Plant Gene Bank of Iran (NPGBI). About 100 different accessions of Ae. cylindrica were estimated in flow cytometry analysis by using Ae. tauschii as  a control sample. All the studied accessions fitted in 4x ploidy level. Chromosomes counts and flow cytometry data led to the same results. There was significant diversity in DNA index among Ae. cylindrica accessions. Cluster analysis was used for the classification of Ae. cylindrica based on their DNA content. Interestingly, accessions with smaller and larger DNA index had smaller and larger chromosome length, respectively. Also, the study of the relationship of DNA content and geographical distribution in Iran showed that there is diversity in DNA content of  Ae. cylindrica  for different areas of Iran.

Keywords


Article Title [Persian]

تعیین سطح پلوئیدی گونه Aegilops cylindrica در ایران از طریق فلوسایتومتری و شمارش کروموزومی

Authors [Persian]

  • بهنام بخشی 1
  • محمد جعفر آقایی 2
  • محمد رضا بی همتا 3
  • فرخ درویش 4
  • عیسی ظریفی 2
1 محقق، باشگاه پژوهشگران جوان، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات
2 استادیار، موسسه تحقیقات اصلاح و تهیه نهال بذر
3 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران
4 استاد، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات
Abstract [Persian]

در این تحقیق از شمارش کروموزومی و روش فلوسیتومتری برای تخمین سطح پلوئیدی نمونه های مختلفAegilops cylindrica Host (2n = 4x = 28, CCDD) موجود در بانک ژن گیاهی ملی ایران استفاده شد. تعداد 100 نمونه  Ae. cylindrica در آنالیز فلوسیتومتری با استفاده از Ae. tauschiiبه عنوان نمونه کنترل مورد استفاده قرار گرفتند. تمام نمونه های مورد بررسی دارای سطح پلوئیدی 4x بودند. نتایج حاصل از فلوسیتومتری و شمارش کروموزومی مشابه بود. تنوع معنی داری در شاخص DNA نمونه های Ae. cylindrica وجود داشت. تجزیه خوشه ای برای طبقه بندی Ae. cylindricaبر اساس محتوای DNA آنها انجام شد. به طور جالبی، نمونه های با شاخص DNA کوچک و بزرگ به ترتیب دارای طول کروموزوم کوتاه و طویل بودند. همچنین، مطالعه رابطه بین محتوای DNA و پراکنش جغرافیایی در ایران نشان داد که برای Ae. cylindrica در مناطق مختلف ایران از لحاظ محتوای DNA تنوع وجود دارد.

Keywords [Persian]

  • Aegilops cylindrica
  • Flow cytometry
  • Ploidy level
  • chromosomes counting
  • Iran
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