CHARACTERIZATION OF GENETIC DIVERSITY IN THREE CHARA L. SPECIES (CHARACEAE) IN IRAN USING RAPD-PCR TECHNIQUE

Document Type : Research Paper

Authors

1 PhD. Student / Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

2 Assistant Professor / Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

3 Associate Professor / Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

Abstract

Species separation in the family Characeae is generally based on morphological characters, but these traits used to identify species are influenced by habitat characteristics. The green algal genus Chara Linnaeus comprises of several species, which are morphologically variable. RAPD-PCR technique was used to characterize genetic diversity in three Chara L. species collected from Iran. Five primers were used in this study. Different loci were detected using a total of 17 samples of Chara vulgaris L., C. gymnophylla A. Braun and C. zeylanica C. L. Willdenow. The present study is the first on the molecular characterization of these species using RAPD-PCR marker in Iran. The method seems to be suitable for characterization of the genus at species level.

Keywords

Article Title [Persian]

تعیین تنوع ژنتیکی سه گونه از جنس کارا در ایران با استفاده از تکنیک رپید

Authors [Persian]

  • سعید افشارزاده 2
  • غلامرضا بلالی 3
1 دانشجوی دکتری / گروه زیست شناسی دانشگاه اصفهان.
2 استادیار / گروه زیست شناسی دانشگاه اصفهان
3 دانشیار / گروه زیست شناسی دانشگاه اصفهان.
Abstract [Persian]

تمایز گونه­ها در خانواده کاراسه عموما بر پایه صفات ریخت­شناسی است، ولی این صفات تحت تاثیر خصوصیات محیطی قرار می­گیرند. جنس کارا شامل تعداد زیادی گونه می­باشد که تغییر­پذیری مورفولوژیکی زیادی را از خود به نمایش می­گذارند. تکنیک مولکولی رپید برای تعیین تنوع ژنتیکی در سه گونه از جنس کارا در ایران مورد استفاده قرار گرفت. در این مطالعه پنج پرایمر استفاده شدند. لوکوس­های مختلفی برای 17 نمونه از گونه­های Chara vulgaris،  C. gymnophylla و C. zeylanica شناسایی شد. مطالعه حاضر برای اولین بار استفاده از تکنیک مولکولی رپید را در تمایز این گونه­ها در ایران شرح می­دهد. به نظر می­رسد این تکنیک برای تمایز گونه­های این جنس مفید و موثر باشد.

Keywords [Persian]

  • Genetic diversity
  • Chara
  • Iran
  • Characeae
  • RAPD-PCR

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The Iranian Journal of Botany
Volume 18, Issue 2 - Serial Number 2
December 2012
Pages 318-326

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History

  • Receive Date: 14 April 2012
  • Revise Date: 09 October 2012
  • Accept Date: 07 November 2012

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