Analysis of phylogenetic relationships of Potamogeton and Stuckenia (Potamogetonaceae) in Iran based on morphological, anatomical and molecular data

Document Type : Research Paper

Authors

1 Department of Biology Education, Farhangian University, P.O. Box 14665-889, Tehran, Iran

2 Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran

3 Forests and Rangelands Research Division, Khuzestan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran

4 Laboratory of Plant Biology and Nature Management (APNA), Department of Biology, Vrije Universiteit Brussel (VUB), Brussels, Belgium

10.22092/ijb.2024.130476

Abstract

Potamogeton and Stuckenia comprise 90 and 7 respectively accepted species worldwide. Because of a wide range of intraspecific morphological variability including extensive phenotypic plasticity and ecological diversity, Potamogeton and Stuckenia are considered notoriously difficult taxa. A total of 100 accessions representing 11 taxa of Potamogeton and three of Stuckenia were collected across Iran. An initial screening based on allele lengths of highly variable cpDNA sequences allowed considering 59 different accessions of one to eight individuals of each species for further morphological, anatomical, and molecular analyses. NrDNA ITS and three plastid regions (rbcL, matK, and trnH-psbA) were employed to reconstruct molecular phylogenies using maximum likelihood and Bayesian inferences. Analyses of nrDNA ITS sequences generated well-resolved tree topology than plastid data. There was some incongruence between nuclear data and concatenated chloroplast marker data (P=0.001). Additional testing of ISSR and SRAP markers for 48 specimens showed higher resolution in species delimitation among linear leaved taxa, though not conclusive. Anatomical features could not separate Iranian species alone and should be used in combination with morphological characters that were highly informative. The results showed that Iranian Potamogeton species contain a rich gene pool due to the specific and diverse geographical conditions of Iran.

Keywords

Article Title [Persian]

تجزیه و تحلیل روابط فیلوژنتیکی Potamogeton و Stuckenia (Potamogetonaceae) در ایران بر اساس داده‌های مورفولوژیکی، تشریحی و مولکولی

Authors [Persian]

  • شبنم عباسی 1
  • سعید افشارزاده 2
  • مهری دیناروند 3
  • لودویک تریست 4
1 گروه آموزش زیست‌شناسی، دانشگاه فرهنگیان، صندوق پستی ۸۸۹-۱۴۶۶۵ تهران، ایران
2 گروه زیست‌شناسی گیاهی و جانوری، دانشکده علوم و فناوری‌های زیستی، دانشگاه اصفهان، اصفهان، ایران
3 مرکز تحقیقات کشاورزی و منابع‌طبیعی خوزستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز، ایران
4 آزمایشگاه زیست‌شناسی گیاهی و مدیریت طبیعت، دانشکده زیست‌شناسی وریج، دانشگاه بروکسل، بلژیک
Abstract [Persian]

Potamogeton و Stuckenia به ترتیب شامل 90 و 7 گونه پذیرفته شده در سراسر جهان هستند. به دلیل طیف وسیعی از تنوعات مورفولوژیکی درون گونه‌ای از جمله انعطاف‌پذیری فنوتیپی گسترده و تنوع زیست محیطی،Potamogeton و Stuckenia  جنس‌های بسیار دشواری در نظر گرفته می‌شوند. در مجموع 100 نمونه به نمایندگی از 11 گونه از Potamogeton و سه گونه از Stuckenia از سراسر ایران جمع‌آوری شدند. غربالگری اولیه بر اساس طول آلل توالی‌هایcpDNA ، امکان در نظر گرفتن 59 نمونه مختلف از یک تا هشت فرد از هر گونه را برای آنالیزهای مورفولوژیکی، تشریحی و مولکولی بیشتر فراهم نمود. توالی‌های هسته‌ای و کلروپلاستی برای بازسازی درختان مولکولی با استفاده از RaxML و رویکرد بیزین استفاده شدند. آنالیزهای nrDNA وضوح بالاتری را نسبت به داده‌های کلروپلاستی، در واگرایی گونه­‌ها نشان دادند. تعدادی ناهماهنگی بین داده‌های هسته‌ای و کلروپلاستی وجود داشت (P=0.001). آنالیزهای اضافه‌تر نشانگرهای ISSR و SRAP برای 48 نمونه، وضوح بالاتری را در تعیین حدود گونه‌ها در میان گونه‌های برگ خطی نشان داد، اگرچه قطعی نیست. ویژگی‌های تشریحی نمی‌توانند گونه‌های ایرانی Potamogeton را به‌تنهایی تفکیک کند و باید در ترکیب با خصوصیات ریخت‌شناسی که بسیار دربردارنده اطلاعات بودند استفاده شود. نتایج نشان دادند که گونه‌های Potamogeton ایرانی با توجه به شرایط خاص و متنوع جغرافیایی ایران دارای خزانه ژنتیکی غنی هستند.

Keywords [Persian]

  • گیاهان آبزی
  • نشانگرهای مولکولی
  • هیبریداسیون
  • ITS
  • درختان مولکولی

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The Iranian Journal of Botany
Volume 29, Issue 2
December 2023
Pages 106-121

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History

  • Receive Date: 28 October 2023
  • Revise Date: 07 December 2023
  • Accept Date: 13 December 2023

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