New insight into the molecular and micromorphological characteristics of Potentilla indica and Potentilla reptans (Rosaceae)

Document Type : Research Paper

Authors

1 Assistant prof. at University of Guilan

2 Faculty of Science, University of Guilan

3 Faculty of Science, Yasouj University

10.22092/ijb.2022.128184

Abstract

The present study investigates the molecular phylogeny and micromorphological characteristics of Potentilla indica (Andrews) Th. Wolf (=Duchesnea indica (Andrews) Focke), and P. reptans L.. For the phylogenetic study, the nrDNA ITS, trnH-psbA, and combined sequence datasets were analyzed using maximum Parsimony (MP), Bayesian inference (BI), and maximum likelihood (ML) methods. For the micromorphological study, epicalyx, calyx, and pedicle characters were examined. Individual and combined analyses of ITS and trnH-psbA data revealed a phylogenetic divergence in the Reptans clade, corresponding to P. reptans and P. indica (groups L and M) in ITS trees, P. reptans, P. hebiichigo Yonek. & H.Ohashi (group O) and P. indica (group N) in plastid and combined trees. In all obtained trees, P. indica showed a sister group relationship with P. reptans. The micromorphological characters of the epicalyx, calyx, and pedicel were found to be taxonomically effective for species separation. Furthermore, the results supported the phylogenetic relationships among the species.

Keywords

Article Title [Persian]

بینش نوین در ویژگی‌های مولکولی و ریزریخت‌شناسی Potentilla indica و Potentilla reptans از تیره گل‌سرخیان

Authors [Persian]

  • مرضیه بیگم فقیر 1
  • شیما پورابراهیم 2
  • ربابه شاهی شاوون 3
1 عضو هیئت علمی دانشگاه گیلان
2 دانشکده علوم، دانشگاه گیلان
3 دانشکده علوم، دانشگاه یاسوج
Abstract [Persian]

در مطالعه­ حاضر، فیلوژنی مولکولی و صفات ریزریخت­شناسی Potentilla indica (Andrews) Th. Wolf (=Duchesnea indica (Andrews) (Focke  و P. reptans L. مورد بررسی قرار گرفت. برای تحلیل­های فیلوژنتیکی، اطلاعات حاصل از توالی­های nrDNA ITS، trnH-psbA به­طور مجزا و ترکیبی با استفاده از روش بیشینه­ی پارسیمونی، تحلیل بایزین و روش بیشینه­ی درست­نمایی انجام شد. ریزریخت­شناسی، صفات کاسبرگ فرعی، کاسبرگ اصلی و دمگل مورد بررسی قرار گرفت. آنالیز داده­های ITS، trnH-psbA و ترکیبی، جدایی فیلوژنتیکی در کلاد Reptans، شاملP. reptans و P. indica (گروه­های L و M) روی درخت ITS، P. reptans L.، P. hebiichigo Yonek. & H.Ohashi (گروه O) وP. indica  (گروه N) روی درختان پلاستیدی و ترکیبی را نشان داد. روی همه درخت­ها،P. indica  به­عنوان گروه خواهری P. reptans قرار گرفت. مشخص شد صفات ریزریخت­شناسی کاسبرگ فرعی، کاسبرگ اصلی و دمگل دارای ارزش تاکسونومیکی و برای جداسازی گونه ها موثر می­باشند. همچنین نتایج حاصل، روابط فیلوژنتیک بین گونه ها را مورد حمایت قرار داد.

Keywords [Persian]

  • Epicalyx
  • calyx
  • pedicle
  • trnH-psbA
  • ITS
  • phylogeny

References

Bentham G. & Hooker J. D. 1865: Genera plantarum. 1, 600-629. - London: Reeve & Co., Williams & Norgate.
Barthlott W., Neinhuis C., Cutler D., Ditsch F., Meusel I., Theisen I., & Wilhelmi H. 1998: Classification and terminology of plant epicuticular waxes. -Bot. J. Linn. Soc. 126: 237-260.‏
Chen X.,·Li J., Cheng T., Zhang W.·Liu Y., Wu P., Yang X., Wang L. & Zhou S. 2020: Molecular systematics of Rosoideae (Rosaceae). -Plant Syst. Evol. 306: 9.
Cho A. 2012: Constructing Phylogenetic Trees Using Maximum Likelihood, Scripps Senior Theses. Paper 46.
Doyle J. J. & Doyle J. L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
Drummond A. J. & Rambaut A. 2007: BEAST: Bayesian evolutionary analysis by sampling trees. -BMC Evol. Biol.: 1-8.
Dobeš C. & Paule J. 2010: A comprehensive chloroplast DNA-based phylogeny of the genus Potentilla (Rosaceae): Implications for its geographic origin, phylogeography and generic circumscription. -Mol. Phylogenet. Evol. 56: 156-175.
Eriksson T., Donoghue M. J. & Hibbs M. S. 1998: Phylogenetic analysis of Potentilla using DNA sequences of nuclear ribosomal internal transcribed spacer (ITS), and its implications for the classification of Rosoideae (Rosaceae). -Plant Syst. Evol. 11: 155-179.
Eriksson T., Hibbs M. S., Yoder A. D., Delwiche C. F. & Donoghue M. J. 2003: The phylogeny of Rosoideae (Rosaceae) based on sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA and the trnL/F region of chloroplast DNA. -Int. J. Plant Sci. 164: 197-211.
Edgar R. C. 2004: MUSCLE: a multiple sequence alignment method with reduced time and space complexity. -BMC bioinform. 5: 113.
Ertter B. & Attar F. 2007: Changes to Potentilla s.l. (Rosaceae) in Flora Iranica. -Rostaniha 7: 299-314.
ErgenAkin Ö., Şenel G. & Akin Y. 2013: Leaf epidermis morphology of some Onosma (Boraginaceae) species from Turkey. -Turk. J. Bot. 37: 55-64.
Faghir M. B., Attar F., Farazmand A., Ertter B. & Eriksen B. 2010: Leaf indumentum types in Potentilla (Rosaceae) and related genera in Iran. -Acta Soc. Bot. Pol. Pol. 79: 139-145.
Faghir M. B., Attar F., Ertter B. & Eriksen B. 2011: Foliar anatomy of the genus Potentilla L. (Rosaceae) in Iran and its taxonomic implication. -IJSTS: 243-256.
Faghir M. B., Attar F., Farazmand A. & Kazempur Osaloo Sh. 2014: Phylogeny of the genus Potentilla (Rosaceae) in Iran based on nrDNA ITS and cpDNA trnL-F sequences with a focus on leaf and style characters’ evolution. -Turk. J. Bot. 38: 417-429.
Faghir M. B., Pourebrahim S. & Attar F. 2018: Morphological phylogenetic analysis of the genera Fragaria and Duchesnea in Iran. -Rostaniha 19: 154-164.
Feng L., Cheng L., Dong Z., Tao D., Barnhart T. E., Cai W. & Liu Z. 2017: Theranostic liposomes with hypoxia-activated prodrug to effectively destruct hypoxic tumors post-photodynamic therapy. -ACS Nano 11: 927-937.‏
Hall B. H., Jaffe A. B. & Trajtenberg M. 2001: The NBER patent citation data file: Lessons, insights and methodological tools. - NBER: w8498. 
Heo K., Kim Y., Icon O., Maki M. & Park M. 2019: The complete chloroplast genome of mock strawberry, Duchesnea indica (Andrews) Th. Wolf (Rosoideae). -Journal Mitochondrial DNA Part B Resources 4: 560-562.
Juzepczuk S. W. 1941: Fragaria L. -In: Komarov V.L. (ed.), Flora USSR, Izd. Akad. Nauk. SSSR, 13: 289-410. Moskva. Leningrad.
Kalkman C. 1988: The phylogeny of the Rosaceae. - J. Linn. Soc. Bot. 98: 37-59.
Khatamsaz M. 1993: Fl. of Iran, Rosaceae. Vol. 6. -Research Institute of Forests and Rangeland.
Kumar V. S. A. & Murugan K. 2015: Taxonomic implications with special references to stomatal variations in Solanum species using light and scanning electron microscope. –Int. J. Appl. Biol. Pharm. 6: 113-125.
Kumar S., Stecher G. & Tamura K. 2016: MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. -Mol. Biol. Evol. 33: 1870-1874.
Kurtto A. & Eriksson T. 2003: Generic delimitation and nomenclatural adjustments in Potentilla (Rosaceae). -Ann. Bot. Fenn. 40: 135-141.
Leht M. & Paal J. 2004: Variation of Potentilla sect. Potentilla (Rosaceae) in Estonia and neighboring countries. - Ann. Bot. Fenn. 41: 53-61.
Miller M. A. 2010: CIPRES Science Gateway survey results. http://www.phylo.org/tools/survey2.html
Naruhashi N., Iwatsubo Y. & Peng C. I. 1999: Cytology, flower morphology, and distribution of Fragaria hayatai Makino (Rosaceae). -Journal of Phytogeography and Taxonomy 47: 139-143.
Naruhashi N. & sagimoto M. 1996: Floral Biology of Duchesnea (Rosaceae). -Plant Species Biol. 11: 173-184.
Ohashi H., & Ohashi K. 2008: New combination in Potentilla with Duchesnea (Rosaceae). -J. Jpn. Bot. 83(1): 60–61.
Panigrahi G. & Dikshit B. K. 1987: Systematics of the genus Potentilla (Rosaceae Juss), its infrageneric classification and evolutionary trends. -Bull. Bot. Surv. India 27: 177-196.
Park J., Heo K., Kim Y., Maki M. & Lee S. 2019: The complete chloroplast genome, Duchesnea chrysantha (Zoll. & Moritzi) Miq. (Rosoideae). -Mitochondrial DNA Part B 4: 951-952.
Posada D. & Buckley T. R. 2004: Model Selection and Model Averaging in Phylogenetics: Advantages of Akaike Information Criterion and Bayesian Approaches Over Likelihood Ratio Tests. -Syst. Biol. 53:793-808.
Persson N. L., Toresen I., Andersen H. L., Smedmark J. E. E. & Eriksson T. 2020: Detecting destabilizing species in the phylogenetic backbone of Potentilla (Rosaceae) using low-copy nuclear markers. -AoB Plants 12: 1-13.
Pourebrahim Sh., Faghir M. B. & Attar F. 2018: Report of new species of Duchesnea indica from Rosaceae for Guilan province. The International Conference on Agricultural Sciences, Medicinal plants and Traditional medicine; Mashhad.
Robertson K. R. 1974: The genera of Rosaceae in the southeastern United States. -J. Arnold Arbor. 55: 303-662.
Ronquist F., Klopfstein S., Vilhelmsen L., Schulmeister S., Murray D. L. & Rasnitsyn A. P. 2012: A total-evidence approach to dating with fossils, applied to the early radiation of the Hymenoptera. -Syst. Biol. 61: 973-999.
Sadeghi S., Faghir M. B., Attar F. & Aalai A. 2021: Achene micromorphology of the genus Potentilla L. (Rosaceae) in Iran and its systematic application. -Turk. J. Bot. 45: 15-42.
Sang T., Crawford D. J., Stuessy T. 1995: Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: Implication for biogeography and concerted evolution. PNAS 92: 6813–6817.
Sang T., Crawford D. J. & Stuessy T. F. 1997: Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). -Am. J. Bot. 84: 1120-1136.
Schulze-Menz G. K. 1964: Rosales. -In: Melcnior H. (ed.), A. Engler's Syllabus der Pflanzenfamilien. Berlin: Borntraeger.
Schönbeck-Temesy E. 1969: Duchesnea (Rosaceae) Flora Iranica (ed. K. H. Rechinger). -Akademische Druck und Verlagsanstalt, Graz.
Schiman-Czeika H. 1969: Rosaceae. Flora Iranica (ed. Rechinger, K. H.). Akademische Druck und Verlagsanstalt, Graz: 78-114.
Shaw J. & Small R. L. 2004: Addressing the “hardest puzzle in American pomology:” Phylogeny of Prunus sect. Prunocerasus (Rosaceae) based on seven noncoding chloroplast DNA regions. Am. J. Bot. 91(6): 985–996.
Smith J. E. 1810: Duchesnea. -In: Rees A. (ed.), The Cyclopedia; or, universal dictionary of arts, sciences, and literature. London.
Soják J. 1987: Notes on Potentilla (Rosaceae) III. Some new taxa from Asia. -Botanische Jahrbücher für Systematik 109: 25-48.
Soják J. 2004: Potentilla L. (Rosaceae) and related genera in the former USSR. Notes on Potentilla XVI. -Bot. Jahrb. Syst. 125: 253-340.
Soják J. 2008: Notes on Potentilla XXI. A new division of the tribe Potentilleae (Rosaceae) and notes on generic delimitations. -Botanische Jahrbücher 127: 349-358.
Soják J. 2012: Potentilla L. (Rosaceae) and related genera in Asia (excluding the former USSR), Africa, and New Guinea-Notes on Potentilla XXVIII. -Plant Divers. Evol. 130: 7-157.
Swofford D. L. 2002: PAUP*: Phylogenetic analysis using parsimony (*and other methods). ver. 4. 0b10. Sinauer.
Töpel M., Lundberg M., Eriksson T. & Eriksen B. 2011: Molecular data and poloidal levels indicate
several putative allopolyploidization events in the genus Potentilla (Rosaceae). -PLoS Currents: Tree of Life 16: 3 RRN1237.
Trifnopoulos J., Nguyen L. T., Haeseler A., Minh B. Q. 2016. W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Res. 44: W232–5. https://doi.org/10.1093/nar/gkw256.
White T. J., Bruns T., Lee S., Taylor J. 1990: Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky J, White T eds. PCR Protocols: A Guide to Methods and Applications. San Diego: Academic Press.
Wolf T. H. 1908: Monographie der Gattung Potentilla. Vol. 16. -Bibliotheca Botanica, Stuttgart.
Zare H., Ramezani Kakroudi E. & Amini T. 2007: A record of Duchesnea indica a (Rosaceae) In Iran, westernmost distributional limit in Asia. -I. J. B. 13: 93-94.
Yonekura K., Ohashi H., & Ohasho K 2008:  Potentilla hebiichigo Yonek. & H. Ohashi (Rosaceae) and Its Distribution. -J. Jpn. Bot. 83: 301–305.
Zhang S. D., Jin J. J., Chen S. Y., Chase M. W., Soltis D. E. Li H.-T., Yang J.-B., Li D.-Z. & Yi T.-S. 2017: Diversification of Rosaceae since the Late Cretaceous based on plastid phylogenomics. -New Phytol. 214: 1355-1367.
 
The Iranian Journal of Botany
Volume 28, Issue 2
December 2022
Pages 77-95

Files

History

  • Receive Date: 05 September 2022
  • Revise Date: 23 October 2022
  • Accept Date: 01 November 2022

Share

How to cite

Statistics