CHROMOSOMAL STUDIES ON FOUR IRANIAN CROUS SPECIES (IRIDACEAE)

Document Type : Research Paper

Authors

1 Department of Biology, Faculty of sciences, University of Tehran

2 Department of Biochemistry, Faculty of Medicine, Medical Sciences University of Tehran

3 Institute of Biochemistry and Biophysics, University of Tehran

Abstract

Four species of Iranian Crocus were chromosomally investigated and their karyotypes were determined: C. sativus (2n=24), C. cancellatus subsp. damascenus (2n=8A), C. gilanicus (2n=24) and C. speciosus subsp. speciosus (2n=12).
On the meiotic studies of pollen mother cells in Crocus sativus unbalanced distribution of chromosomes in two poles of cells and chromosomic lagging were frequently observed. Karyological studies on the meristematic cells of neoformed root tips in explant of saffron corm showd cells with 2n=16 chromosomes.
 

Keywords

Article Title [Persian]

مطالعات کروموزومی چهار گونه زعفران

Authors [Persian]

  • حسن ابراهیم زاده 1
  • عذرا صبورا 1
  • محمدرضا نوری دلوئی 2
  • سید محمود غفاری 3
1 گروه زیست شناسی، دانشکده علوم، دانشگاه تهران
2 گروه بیوشیمی، دانشکده داروسازی، دانشگاه علوم دارویی تهران
3 موسسه بیوشیمی و بیوفیزیک دانشگاه تهران
Abstract [Persian]

چهار گونه زعفران ایران از نظر کروموزومی مورد بررسی قرار گرفت و کاریوتایپ آنها تعیین شد. 2n در این
گونه‌ها برابر با 24 در زعفران مزروعی  ( Corcus sativus) ،  در زعفران مشبک زیر گونه دمشق( 8A (Crocus cancellatus subsp. damascenes،  در زعفران گیلان 24 (Crocus gilanicus)    و 12 در زعفران زیبا زیر گونه زیبا، (Crocus speciosus subsp. speciosus )  بود.
در بررسی تقسیم میوز سلولها مادر دانه‌گرده در زعفران مزروعی، توزیع نامتعادل کروموزوم‌ها در قطبین سلول و تاخیر کروموزومی به فراوانی مشاهده شد. بررسی کاریولوژیکی ریشه‌های نو پدید در قطعه جدا کشت بنه‌های زعفران مزروعی وجود سلولهای با 2n=16 را نشان داد.

Keywords [Persian]

  • No keywords

References

Aghamohammadi, Z. 1976: Investigation of vegetative propagation, establishment of variation by mutagen agents and study of pollen stainability and pollen germination in relation to sterility of saffron (Crocus sativus L.). Thesis of MS. -Faculty of Sciences, University of Tehran.
Bidault, M. 1971: Variation et Speciation Chez Les Vegetaux Superieurs. Notions Fondamentales de Systematique Moderne. -Paris.
-, B. Mathew and C. J. Marchant, 1973: Chromosome counts in the genus Crocus (Iridaceae). Kew Bull., 28, 451-464.
Brighton, C. A., 1977 a: Cytological prolems in the genus Crocus, II. Crocus cancellatus aggregate. -Kew Bull, 32-33-45
- , 1977 b: Cytology of Crocus satuvus L. (Iridaceae) and its allies. -PI. Syst. Evol., 128: 137-157.
- , 1983. A detailed study of Crocus speciosus and its ally C. pulchellus (Iridaceae). -PI. Syst. Evol., 142, 187-206.
Chichiricco G., 1984. Karyotype and meiotic behavior of the triploid Crocus sativus L. -Cariologia, 37(3): 233-239.
- , 1987:    Megasporogenesis and development of embryo sac in Crocus sativus L. -Cariologia, 40(1-2), 59-69.
- and M. Grilli Cariola, 1982: Germination and variability of the pollen of Crocus sativus L. -G. Bot. ItaI., 116: 167-173.
 
-, - 1986: Crocus sativus pollen germination and pollen tube growth in vitro and after intraspecific and interspecific pollination. -Can. J. Bot.: 64, 2774-2777.
Estilai A., and Aghamohammadi Z. 1977: Pollen stainability and pollen germination in relation to sterility of saffron (Crocus sativus L.). -Bull. Fac. Sci. Tehran. Univ. 9(1), 10-15.
Ghaffari, S. M. 1986. Cytogenetic studies of cultivated Crocus sativus (Iridaceae). -PI. Syst. Evol.: 153, 199-204.
- , 1991. Cytologically studies on C. sativus L. from Iran. Thesis, -Institute of Biochemistry and Biophysics, Tehran University.
                                     Himmerbaur W., 1926: Zur Entwicklungsgeschichte von Crocus sativus L. Festschrift, Tschirch Taunitz, Leipzig, 335-350.
Karasawa K., 1933: On the triploidy of Crocus sativus L. and its high sterility. -Jap. J. Genet., 9: 6-8.
- , 1940: Karyological studies in Crocus. II. -Jap. J. Bot., 2: 129-140.
- , 1943: Karyological studies on Crocus. III. -Jap. J. Bot., 12: 457-503.
Linsmaier G. M. and F., Skoog, 1965: Organic growth factor requirements of tobacco tissue cultures. –PhysiologiaPlantarum, vol. 18: 100-127.
Mathew B., 1982: The Crocus. A revision of the genus Crocus (Iridaceae). -London, Batsford Ltd.
- and C. A. Brighton, 1976: Notes on Crocus gilanicus. -Iran. Journ. Bot., 1: 19-21.
Mobayyen S., 1974. Plants of Iran, Vascular Plants. vol. 1, Gymmnosperms, Monocotyledons. -Tehran, Tehran University.
Morinaga T. and E., Fukushima 1931: Chromosome numbers of cultivated plant. III. -Bot. Mag. (Tokyo), 45, 531: 140-145.
Noori-Daloii M. R., 1975: Cytogenetical studies of Crocus sativus L. and investigation of its planting possibility in other region of Iran. Thesis of MS, Faculty of Sciences, -Tehran University.
Pathak G. N., 1940: Studies in the cytology of Crocus. -Ann. Bot, new series 4: 227-256.
Philips R. L., 1981: Plant cytogenetics in staining procedure. U. S. A, Clark G. (ed). -Wilkins.
Pogliani M. and F. D. Grooso, 1971: Studio cariologico di Crocus sativus. -Inform. Bot. Ital. 4: 25-29.
Rudall Paula J., S. J. Owens and A. Y. Kenton, 1984: Embryology and breeding systems in Crocus (Iridaceae): A study in causes of chromosome variation. -PI. Syst. Evoi. 148: 119-134.
Stebbins G. L., 1971: Chromosomal evolution in Higher Plants, Contemporary Biology Series. –EdwardArnold, London.
Wendelbo P. and Mathew B., 1975: Iridaceae in K H. Rechinger Fl. Iranica. no. 112. -Graz.
- , 1997: Tulips and Irises of Iran, their Relatives. -Botanical Institue of Iran, Tehran.

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  • Receive Date: 15 December 2015
  • Accept Date: 15 December 2015

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