CYTOPHOTOMETRIC ESTIMATION OF 4C DNA CONTENT AND KARYOTYPE ANALYSIS IN TEN CULTIVARS OF TRIGONELLA FOENUM-GRAECUM

Document Type : Research Paper

Authors

Cytogenetics Laboratory, Regional Plant Resource Centre, Bhubaneswar 751015, Orissa, India

Abstract

 
Estimation of nuclear DNA content through cytophoto-densitometer in somatic cells and karyotype analysis were carried out for the first time in ten cultivars of fenugreek (Trigonella foenum-graecum). Somatic chromosome number is reported 2n=16 in all the cultivars. Detailed karyotype analysis revealed cultivar specific chromosomal characteristics and minute structural alterations in chromosomes. The 4C DNA content varied significantly from 7.23 pg in the cultivar TG-103 to 9.12 pg in  TG-09. Significant variations in the genome length, volume and total form percentage were noted at cultivar level. A positive significant correlation coefficient revealed interdependence between the chromosome volume and nuclear DNA content of the cultivars. Significant variations in 4C nuclear DNA content among different cultivars despite the same somatic chromosome number suggest a changes in the repetitive DNA sequences of the genomes.

Keywords


Article Title [Persian]

تخمین سیتوفتومتری4C DNA و تجزیه کاریوتیپ ده رقم شنبلیله

Authors [Persian]

  • آنات باندهو داس
  • پرماناندا داس
  • اس موهانتی
آزمایشگاه سیتوژنتیک مرکز تحقیقات گیاهی بوبانسوار اوریسای هند
Abstract [Persian]

تخمین محتوای DNA هسته سلولهای سوماتیک از طریق سیتوفتومتری و تجزیه کاریوتیپ ده رقم شنبلیله (Trigonella foenum-graecum) برای اولین بار انجام شد. تعداد کروموزومهای سوماتیک 16=n2 برای همه رقمها گزارش می‌شود.  جزییات کاریوتیپ در ارقام مختلف، تغییرات ساختاری کمی را نشان می‌دهد. محتوی 4C DNA از pg 23/7 در یکی از ارقام تا pg 12/9 در رقم دیگری تفاوت می‌کند. تغییرات معنی‌داری در طول و حجم ژنوم و همچنین درصد شکل کل (TF%) در سطح ارقام دیده شد. ضریب همبستگی مثبت بین حجم کروموزوم و محتوای DNA هسته در ارقام مشاهده گردید. گوناگونی معنی‌دار در مقدار 4C DNA بین ارقام مختلف، علیرغم برابری تعداد کروموزومهای ثابت، نشان دهنده تغییر در توالی DNA ژنوم است.


Keywords [Persian]

  • . fenugreek
  • nuclear DNA
  • Karyotype
  • Trigonella
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