CYTOPHOTOMETRIC ESTIMATION OF 4C DNA CONTENT AND KARYOTYPE ANALYSIS IN TEN CULTIVARS OF TRIGONELLA FOENUM-GRAECUM

Document Type : Research Paper

Authors

Cytogenetics Laboratory, Regional Plant Resource Centre, Bhubaneswar 751015, Orissa, India

Abstract

 
Estimation of nuclear DNA content through cytophoto-densitometer in somatic cells and karyotype analysis were carried out for the first time in ten cultivars of fenugreek (Trigonella foenum-graecum). Somatic chromosome number is reported 2n=16 in all the cultivars. Detailed karyotype analysis revealed cultivar specific chromosomal characteristics and minute structural alterations in chromosomes. The 4C DNA content varied significantly from 7.23 pg in the cultivar TG-103 to 9.12 pg in  TG-09. Significant variations in the genome length, volume and total form percentage were noted at cultivar level. A positive significant correlation coefficient revealed interdependence between the chromosome volume and nuclear DNA content of the cultivars. Significant variations in 4C nuclear DNA content among different cultivars despite the same somatic chromosome number suggest a changes in the repetitive DNA sequences of the genomes.

Keywords

Article Title [Persian]

تخمین سیتوفتومتری4C DNA و تجزیه کاریوتیپ ده رقم شنبلیله

Authors [Persian]

  • آنات باندهو داس
  • پرماناندا داس
  • اس موهانتی
آزمایشگاه سیتوژنتیک مرکز تحقیقات گیاهی بوبانسوار اوریسای هند
Abstract [Persian]

تخمین محتوای DNA هسته سلولهای سوماتیک از طریق سیتوفتومتری و تجزیه کاریوتیپ ده رقم شنبلیله (Trigonella foenum-graecum) برای اولین بار انجام شد. تعداد کروموزومهای سوماتیک 16=n2 برای همه رقمها گزارش می‌شود.  جزییات کاریوتیپ در ارقام مختلف، تغییرات ساختاری کمی را نشان می‌دهد. محتوی 4C DNA از pg 23/7 در یکی از ارقام تا pg 12/9 در رقم دیگری تفاوت می‌کند. تغییرات معنی‌داری در طول و حجم ژنوم و همچنین درصد شکل کل (TF%) در سطح ارقام دیده شد. ضریب همبستگی مثبت بین حجم کروموزوم و محتوای DNA هسته در ارقام مشاهده گردید. گوناگونی معنی‌دار در مقدار 4C DNA بین ارقام مختلف، علیرغم برابری تعداد کروموزومهای ثابت، نشان دهنده تغییر در توالی DNA ژنوم است.


Keywords [Persian]

  • . fenugreek
  • nuclear DNA
  • Karyotype
  • Trigonella

References

Anonymous. 1976: In The Wealth of India. Vol 10, pp 298-306. Publication and Information Directorate (CSIR). -New Delhi.
Bir S. S. and Kumari S. 1980: Cytological evolution of the leguminous flora of the Punjab plain. In Recent Researches in Plant Sciences. pp 251-60. Edited by S. S. Bir. -Kalyani Publishers, Ludhiana, India.
Chattopadhyay D. & Sharma A. K. 1990: Chromosome studies and microspectrophotometric estimation of nuclear DNA in different strains of Coriandrum sativum L. -Cytobios 64: 43-51.
Das A. B. & Mallick R. 1989a: Variation in karyotype and nuclear DNA content in different varieties of Foeniculum vulgare Mill. -Cytologia 54:129-34.
Das A. B. & Mallick R. 1989b: Varietal differences in 4C DNA content and chromosome characteristics of Coriandrum sativumL. -Cytologia 54. 609-16.
Das A. B. & Mallick R: 1993a: Nuclear DNA and chromosomal changes within the tribe Ammineae. -Cytobios 74: 197-207.
Das A. B. & Mallick R. 1993b: Karyotype diversity and interspecific 4C DNA variation in Bupleurum. -Biologia Pl. 35 335-63.
Das A. B. & Chaterjee A. 1994. Analysis of genome in relation with seed protein diversity of Cassia using polyacrylamide gel-electrophoresis. -Bangladesh Jour. Bot. 23 167-174.
Das A. B. & Das P. 1994: Estimation of 4C DNA content and karyotype analysis in edible varieties of banana (Musa acuminata). -Cytobios 78: 213-20.
Das A. B. & Das P. .1994: Estimation of nuclear DNA and karyotype analysis in nine cultivars of Musa. Cytobios 90: 181-192.
Das A. B., Pattanaik M., Thungaraj T. & Das P. 1997. Cytophotometric estimation of nuclear 4C DNA content and karyotype analysis of eight cultivars of Trigonella foenum-graecum. -Cytobios 91:171-179.
Dhillon S. S. & Miksche J. P. 1982: DNA content and heterochromatin variation in various tissues of peanut (Arachis hypogaea). -Am. J. Bot. 69:219-26.
Frahm-Leliveld J. A. 1957: Observations cytologiques sur quelques Legumineuses tropicales et subtropicales. -Rev. Cytol. Bioi. Veg. 18:273-87.
Hammatt N., Blackhall N. W. & Davey M. R. 1991. Variation in the DNA content of Glycine species. -J. Exp. Bot. 42: 659-65.
Ignacimuthu S. & Babu C. R. 1988. Nuclear DNA and RNA amounts in the wild and cultivated Urd and Mung beans and their M1 plants. -Cytologia 53: 535-41.
Lavania U. C. & Sharma A. K. 1980: Giemsa C-banding, somatic association and orientation of interphase chromosomes in Trigonella foenum-graecum L. -Caryologia 33:17-23.
Maxted N., Callimassia M. A. & Bennett M. D. 1991: Cytotaxonomic studies of Eastern Mediterranean Vicia species (Leguminosae). -Plant Syst. Evol. 177: 221-34.
Mukherjee S. & Sharma A. K 1986: In situ nuclear DNA quantitation in organs of different strains of Cicer arietinum L. -Cytobios 48: 151-6.
Ohri D., Kumur A. & Pal M. 1986: Correlations between 2C DNA values & habitat in Cassia (Leguminosae: Caesalpinioideae). -Plant Syst. Evol. 153: 223-7.
Parida A., Raina S. N. & Narayan R. K. J. 1990: Quantitative DNA variation between and within chromosome complements of Vigna species (Fabaceae). -Genetica 82: 125-33.
Price H. J. 1976: Evolution of DNA content in higher plants. -Bot. Rev. 42: 27-52.
Price H. J., Bachman K., Chambers K. L. & Riggs J. 1980: Detection of interspecific variation in nuclear DNA content in Microseris douglasii. -Bot. Gaz. 141: 195-8.
Raghuvanshi S. S. & Singh A. K. 1974a: A possible mutagenic effect of colchicine in Trigonella foenum-graecum. -Caryologia 39: 73-6.
Raghuvanshi S. S. & Singh A. K. 1974b: Studies on the effect of gamma rays on Trigonella foenum-graecum. -Cytologia 39: 473-82.
Raina S. N & Bisht M. S. 1988: DNA amounts and chromatin compactness in Vicia. Genetica 77: 65-77.
Sharma A. K. & Sharma A. 1980: Chromosome techniques: theory and practice. Third edition -Butterworths, London.
Singh A. & Roy R. P. 1970: Karyotype studies in Trigonella, Indigoferaand Phaseolus.
-Nucleus 13: 41-54.
Sokal P. R. & Rohlf F. J. 1973: Introduction to Biostatistic. -Freeman, San Franscisco.
Van't hof J. 1965: Relationships between mitotic cycle duration, S period duration and the average rate of DNA synthesis in the root meristem cells of several plants. -Expl Cel1 Res. 39: 48.
Yamaguchi Y. & Tsunoda S. 1969: Nuclear volume, nuclear DNA content and radiosensitivity in Brassica and allied genera. -Jap. J. Breed. 19: 350-6.
 

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  • Receive Date: 08 December 2015
  • Accept Date: 08 December 2015

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