گونه Oxytropis azizii به‌عنوان ترکیب جدیدی براساس شواهد مولکولی و ریخت‌شناسی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران

2 موسسه تحقیقات جنگل‌ها و مراتع کشور، تهران، ایران

3 دانشگاه علوم پزشکی تبریز، تبریز، ایران

چکیده

براساس شواهد مولکولی و ریخت­شناسی، نتایج ما به‌صراحت نشان می‌دهند که گونه‌ی Astragalus azizii به‌عنوان عضوی از جنس Oxytropis رده‌بندی می‌شود و گونه‌ای مجزا و خویشاوند نزدیکی از O. lapponica است. در حین بررسی‌های ما برروی بخش Brachylobium از جنس گون، نمونه‌های تیپ و ایزوتیپ گونه‌ی A. azizii آنالیز شد و درخت تبارزایی حاصل از توالی‌های nrDNA ITS نشان داد که این گونه در میان گونه‌های Oxytropis قرار می‌گیرد. تشریح گل بادقت انجام گرفت و زائده‌ی نوک تیز انتهایی برروی گلبرگ ناو مشاهده شد (صفت شاخص جنس Oxytropis).

کلیدواژه‌ها


Bidarlord, M., Jamzad, Z., Jalili, A., Mehravaran, D., & Talaei, R. 2022: Conservation status of the alpine species, Astragalus azizii Maassoumi. -Iran Nature 7(4): 139–143. https://doi.org/10.22092/irn.2022.358777.1466 (in Persian).
Candolle, A.P.De. 1802: Astragalogia. -Parisiis, sumptibus Joann. Bapt. Garnery (Typis Didot Junioris).
Doyle, J. J. & Doyle, J. L. 1987: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. –Phytochem. Bull. 19: 11–15.
Darriba, D., Posada, D., Kozlov, A.M., Stamatakis, A., Morel, B., & Flouri, T. 2020: ModelTest-NG: A New and Scalable Tool for the Selection of DNA and Protein Evolutionary Models. –Mol. Biol. Evol. 37 (1): 291–294, https://doi.org/10.1093/molbev/msz189
Douzery, E.J.P., Pridgeon, A.M., Kores, P., Linder, H.P., Kurzweil, H., & Chase, M.W. 1999: Molecular phylogenetics of Diseae (Orchidaceae): a contribution from nuclear ribosomal ITS sequences. –Am. J. Bot. 86(6): 887–899. https://doi.org/10.2307/2656709  
Duan, L., Li, S.J., Su, C., Sirichamorn, Y., Han, L.N., Ye, W., Lôc, P.K., Wen, J., Compton, J.A., Schrire, B., Nie, Z.L., & Chen, H.F. 2021: Phylogenomic framework of the IRLC legumes (Leguminosae subfamily Papilionoideae) and intercontinental biogeography of tribe Wisterieae. –Mol. Phylogenet.  Evol. 163: 107235. https://doi.org/10.1016/j.ympev.2021.107235
Edler, D., Klein, J., Antonelli, A., & Silvestro, D. 2021: raxmlGUI 2.0: A graphical interface and toolkit for phylogenetic analyses using RAxML. -Methods Ecol. and Evol. 12: 373–377. https://doi.org/10.1111/2041-210X.13512
Hall, T.A. 1999: BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. -Nucleic Acids Symp. Ser. 41: 95–98.
Katoh, K., Rozewicki, J., & Yamada, K.D. 2019: MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. -Brief. Bioinformatics 20(4): 1160–1166, https://doi.org/10.1093/bib/bbx108
Kazempour-Osaloo, S., Maassoumi, A.A., & Murakami, N. 2003: Molecular systematics of the genus Astragalus L. (Fabaceae): Phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast gene ndhF sequences. –Pl. Syst. Evol. 242 (1): 1–32.
Kazempour-Osaloo, S., Maassoumi, A.A., & Murakami, N. 2005: Molecular systematics of the Old World Astragalus (Fabaceae) as inferred from nrDNA ITS sequence data. -Brittonia 57(4): 367–381. https://doi.org/10.1663/0007-196x(2005)057[0367:msotow]2.0.co;2
Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P., & Drummond, A. 2012: Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. -Bioinformatics (Oxford, England), 28(12): 1647–1649. https://doi.org/10.1093/bioinformatics/bts199  (https://www.geneious.com).
Kozlov, A.M., Darriba, D., Flouri, T., Morel, B., & Stamatakis, A. 2019: RAxML-NG: a fast, scalable and user-friendly tool for maximum likelihood phylogenetic inference. -Bioinformatics 35(21): 4453–4455. https://doi.org/10.1093/bioinformatics/btz305
Langmead, B., & Salzberg, S.L. 2012: Fast gapped-read alignment with Bowtie 2. -Nature Methods 9(4): 357–359. https://doi.org/10.1038/nmeth.1923
Letunic, I., & Bork, P. 2021: Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. -Nucleic Acids Res. 49 (W1): W293–W296. https://doi.org/10.1093/nar/gkab301
Li, Q.Q., Niu, Y., Zhang, Z.P., Wen, J., & Liao, C.Y. 2025: Comparative chloroplast genome analyses of Oxytropis DC. species: new insights into genome evolution and phylogenomic implications. –Front. Pl.Sci., 16: 1645582. https://doi.org/10.3389/fpls.2025.1645582
Maassoumi, A.A. 1989: Revision of Astragalus L. sect. Hemiphaca Bunge (Leguminosae) in Iran. In: Mitteil. der Bot. Staatss. München 28: 501–511. OCLC: 1590119
Maassoumi, A.A. 1990: Illustrated guide to the genus Astragalus in Iran. vol. 1. -Tehran, Research Institute of Forests & Rangelands.
Maassoumi, A.A. 2013: A contribution to the taxonomy of the genus Oxytropis (Fabaceae) in Iran. -Iran. J. Bot. 19(1): 1–28. https://doi.org/10.22092/ijb.2013.2980  
Maassoumi, A.A. 2018: Astragalus III. In: Assadi M. & Maassoumi A.A. (eds.). Flora of Iran, no. 145: 20–67. -Tehran, Research Institute of Forests & Rangelands.
Maassoumi, A.A. 2023: Oxytropis DC. (Fabaceae). In: Assadi, M. & Maassoumi, A.A. (eds.). Flora of Iran, no. 177: 135–185. -Tehran, Research Institute of Forests & Rangelands.
Malyshev, L.I. 2008: Phenetics of subgenera and sections in the genus Oxytropis DC. (Fabaceae) bearing on ecology and phylogeny. –Contemp. Probl.  Ecol. 1: 400–440.
Miller, M.A., Pfeiffer, W., & Schwartz, T. 2010: Creating the CIPRES Science Gateway for inference of large phylogenetic trees. –Proc.  Gateway Comp. Environ. Workshop (GCE), New Orleans, LA. Pp: 1–8. https://doi.org/10.1109/GCE.2010.5676129  
Minh, B.Q., Schmidt, H.A., Chernomor, O., Schrempf, D., Woodhams, M.D., von Haeseler, A., & Lanfear, R. 2020: IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era. –Mol. Biol.Evol. 37 (5): 1530–1534. https://doi.org/10.1093/molbev/msaa015
Moghaddam, M., & Kazempour-Osaloo, S. 2020: Extensive survey of the ycf4 plastid gene throughout the IRLC legumes: Robust evidence of its locus and lineage-specific accelerated rate of evolution, pseudogenization and gene loss in the tribe Fabeae. -PLOS ONE, 15 (3): e0229846. https://doi.org/10.1371/journal.pone.0229846
Podlech, D. & Zarre, S. 2013: Taxonomic Revision of the Genus Astragalus L. (Leguminosae) in the Old World, vol. 1–3 -Vienna, Naturhistorisches Museum Wien.
Rambaut, A. 2018: Figtree version 1.4.4. Institute of Evolutionary Biology, University of Edinburgh, Edinburgh. http://tree.bio.ed.ac.uk/software/figtree/
Ronquist, F., Teslenko M., van der Mark, P., Ayres, D. L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A., & Huelsenbeck, J.P. 2012: MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. -Syst. Biol.61(3): 539–542, https://doi.org/10.1093/sysbio/sys029
Shahi-Shavvon, R., Kazempour-Osaloo, S., Maassoumii, A.A., Moharrek, F., Karaman-Erkul, S., Lemmon, A.R., Lemmon, E.M., Michalak, I., Muellner-Riehl, A.N. & Favre, A. 2017: Increasing phylogenetic support for explosively radiating taxa: The promise of high-throughput sequencing for Oxytropis (Fabaceae). –J. Syst.  Evol. 55: 385–404. https://doi.org/10.1111/jse.12269
Stamatakis, A. 2014: RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. -Bioinformatics 30 (9): 1312–1313, https://doi.org/10.1093/bioinformatics/btu033
Thiers, B.M. 2023: Index Herbariorum. [online] https://sweetgum.nybg.org/science/ih/ Retrieved 20 May 2025.
Vassilczenko, I.T. 1984: Oxytropis DC. In: Rechinger K.H. (ed.). Flora Iranica, vol. 157: 101–164. -Graz, Akademische Druck-Universität.
Wojciechowski, M.F. 2005: Astragalus (Fabaceae): A molecular phylogenetic perspective. -Brittonia 57: 382–396. https://doi.org/10.1663/0007-196X(2005)057[0382:AFAMPP]2.0.CO;2