TY - JOUR ID - 4157 TI - RAPD ANALYSIS OF GENETIC VARIATION WITHIN AND AMONG NATURAL POPULATIONS OF TWO SPECIES OF DIANTHUS L. (CARYOPHYLLACEAE) IN NE IRAN JO - The Iranian Journal of Botany JA - IJB LA - en SN - 1029-788X AU - Behroozian, Maryam AU - Jafari, Azarnoosh AU - Farsi, Mohammad AD - Departemt of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran AD - Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. Y1 - 2013 PY - 2013 VL - 19 IS - 2 SP - 194 EP - 201 KW - Dianthus KW - Caryophyllaceae KW - Iran KW - RAPD markers KW - Genetic variation DO - 10.22092/ijb.2013.4157 N2 - In this study we investigated the genetic variation, using RAPD data in 69 individuals of nine natural populations representing two species of Dianthus L. growing in Northeast of Iran. Eleven RAPD primers generated 111 polymorphic DNA bands. The percentage of polymorphic bands, Nails genetic diversity (h), as well as Shannon index (I), were assessed. A dendrogram based on UPGMA segregated examined populations into two main clusters matching the two species and the two subspecies of Dianthus polylepis subsp. polylepis and D. polylepis subsp. D. binaludensis were to each other. AMOVA showed 82% and 18% of the variation within and among populations respectively. Furthermore, principal coordinate analysis (PCA) based on a Euclidean metric revealed that three populations of D. crinitus subsp. turcomanicus were genetically different from six populations of D. polylepis. According to our results, geographical distances, mating system and gene flow have important effects on genetic polymorphism of populations. UR - https://ijb.areeo.ac.ir/article_4157.html L1 - https://ijb.areeo.ac.ir/article_4157_e870bc65fc1886877902c83322a46d7b.pdf ER -