MOLECULAR AUTHENTICATION OF THYMUS PERSICUS BASED ON NRDNA ITS SEQUENCES DATA

Document Type : Research Paper

Authors

1 Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

2 Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

3 Department of Botany, Research Institute of Forests and Rangelands, Tehran, Iran

Abstract

Thymus persicus is an endemic species of the genus Thymus (Lamiaceae) which grows in Zanjan and West Azerbaijan (Takab) provinces of Iran. Among the Iranian Thymus species, T. persicus is well differentiated by having the smallest leaf width among Thymus species with long non-glandular and short glandular hairs. In order to evaluate the phylogenetic relationships of the T. persicus and T. marandensis Jamzad, the recently and assumed related species to T. brachychilus Jalas and T. leucotrichus Hal., a molecular analysis based on nrDNA ITS sequences of 25 accessions belonging to 19 species of Thymus together with representatives of genera Origanum, Thymbra, Satureja, Micromeria, Gontscharovia, Ziziphora, Saccocalyx and Zataria was performed. The results of phylogenetic analysis showed that the genus Thymus is paraphyletic when the monotypic genus Saccocalyx is included and its sister relationship with Origanum was confirmed. While the phylogenetic position of T. persicus among the other taxa of Thymus is appeared unresolved. T. marandensis turned out to be the sister to a group of Thymus species including T. carmanicus, T. migricus, T. pubescens, T. trautvetteri and T. daenensis.

Keywords

Article Title [Persian]

شناسایی و تایید مولکولی آویشن ایرانی (Thymus persicus) بر اساس توالی‌های nrDNA ITS

Authors [Persian]

  • علی سنبلی 1
  • محمد حسین میرجلیلی 2
  • زیبا بختیار 2
  • زیبا جم زاد 3
1 استادیار گروه بیولوژی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی، تهران.
2 استادیار گروه کشاورزی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی، تهران.
3 استاد بخش تحقیقات گیاه شناسی، موسسه تحقیقات جنگلها و مراتع، تهران
Abstract [Persian]

آویشن ایرانی (Thymus persicus) یکی از گونه­های انحصاری جنس Thymus است که در استانهای زنجان و آذربایجان غربی (تکاب و حومه) ایران رویش دارد. این گونه با داشتن برگ­های باریک خطی شکل با کرک­های غیرغده­ای بلند به همراه کرکهای غده­ای تک سلولی کوتاه در بین سایر گونه­های جنس آویشن به خوبی متمایز است. برای تعیین جایگاه تبارزایشی گونه آویشن ایرانی و روابط خویشاوندی آن با سایر گونه­ها و تایید مولکولی این گونه، 25 نمونه متعلق به 19 گونه از جنس Thymus به همراه گونه­هایی از جنس­های خویشاوند Origanum، Satureja، Thymbra، Micromeria، Ziziphora، Gontscharovia ، Saccocalyx و Zataria انتخاب و آنالیز تبارزایشی بر اساس توالی­های nrDNA ITS انجام شد. کلادوگرام حاصل از آنالیز بیزین نشان داد که جنس آویشن با در نظر گرفتن جنس Saccocalyx پیرا تبار است و رابطه خویشاوندی خواهری آن با جنس Origanum تایید شد. گونه T. persicus با وجود صفات متمایز کننده خاص به همراه سایر گونه­های این جنس در کلاد تک تبار Thymus بصورت پلی تومی و حل نشده ظاهر شد. گونه
 T. marandensis به عنوان یک گونه خواهری با برخی از گونه های این جنس از جمله T. daenensis، T. migricus، T. pubescens، T. trautvetteri و T. carmanicus قرار گرفت.

Keywords [Persian]

  • Thymus persicus
  • Thymus marandensis
  • Lamiaceae
  • phylogeny
  • Taxonomy
  • DNA barcoding

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The Iranian Journal of Botany
Volume 19, Issue 2 - Serial Number 2
December 2013
Pages 179-185

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History

  • Receive Date: 30 December 2013
  • Accept Date: 30 December 2013

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