Phylogenetic relationships within Ferula sect. Merwia (Apiaceae- Ferulinae) inferred from nrDNA and cpDNA markers

Document Type : Research Paper

Author

Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

10.22092/ijb.2023.363199.1429

Abstract

Ferula L., as a large genus in the Umbelliferae with 180-185 species, is distributed only in the Old World. There are 25 mostly medicinal species within the Ferula sect. Merwia, of which 14 species are distributed in Iran. A molecular phylogenetic study of the Iranian species using two nrDNA (ITS and ETS) and two cpDNA (rpL32―trnL(UAG) and rps16― trnK(UUU)) markers was undertaken to determine the phylogenetic relationships among species and evaluate their potential value in taxonomic treatment of the genus. The Maximum Likelihood and Bayesian Inferences trees revealed that the section contained three geographically separated subclades. Also, F. assa-foetida has appeared as two geographic ecotypes in Iran; one group contains the populations distributed in lower altitudes in the Centre of Iran and the other includes the populations from higher altitudes in Zagros Mt. in the south of Iran. Ferula alliacea is placed close to the eastern elements of the sect. Merwia. Similarly, the position of F. lutensis as well as F. latisecta is confirmed within the section. The used cpDNA regions could not define the boundaries of infraspecific taxa of Ferula species such as F. assa-foetida within the sect. Merwia, while they contributed to separating different species of other sections of the genus and also allied genera (Leutea). Accordingly, the most informative region was the ETS nrDNA which may contain more nucleotide substitution. In our analyses, Leutea turcomanica is located close to the other Leutea species.

Keywords

Article Title [Persian]

تعیین روابط فیلوژنتیک درون بخش Merwia از جنس Ferula (Apiaceae- Ferulinae) توسط نشانگرهای مولکولی هسته‌ای و کلروپلاستی

Author [Persian]

  • مهرنوش پناهی
موسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
Abstract [Persian]

جنس کما (Ferula) یکی از بزرگترین جنس‌های تیره چتریان، با حدود 180-185 گونه منتشر شده در دنیای قدیم است. بخش Merwia در جنس Ferula طبق مطالعات اخیر، با 25 گونه به عنوان یک بخش حاوی گونه‌های مهم دارویی معرفی شده است. 14 گونه از این بخش در ایران گسترش دارد که توسط داده‌های مولکولی هسته‌ای (دو ناحیه ITS و ETS) و کلروپلاستی (دو ناحیه فضای بین ژنی rpL32―trnL(UAG) و trnK(UUU)― rps16) به منظور تعیین موقعیت دقیق تاکسونومیک و روابط فیلوژنتیکی گونه‌های درون بخش مورد بررسی قرار گرفت. درختان حاصل از آنالیز حداکثر شباهت (ML) و بایزین (BI) نشان داد که این بخش دربرگیرنده سه گروه متمایز جغرافیایی است که از نظر خواص دارویی نیز تا حدودی متفاوتند. همچنین گونه F. assa-foetida به صورت دو اکوتیپ در ایران ظاهر می‌شود؛ یک گروه جمعیت‌های بخش مرکز ایران پراکنده در ارتفاعات پایین، و گروهی دیگر جمعیت‌های مربوط به ارتفاعات بالاتر در ناحیه زاگرس (جنوب ایران) می‌باشد. گونه F. alliacea در گروه عناصر شرقی بخش Merwia قرار می‌گیرد. جایگاه دو گونه F. lutensis و F. latisecta درون بخش Merwia تعیین می‌گردد. در این مطالعه نواحی کلروپلاستی نتوانست تفکیک افراد فروگونه‌ای بطور مثال در گونه F. assa-foetida در این بخش را مشخص نماید در حالی که برای تفکیک سایر گونه‌های این جنس و جنس نزدیک (Leutea) موثر بودند. علاوه بر این، ناحیه هسته‌ای  ETSبه عنوان بهترین ناحیه حاوی اطلاعات مولکولی مشخص می‌گردد. موقعیت تاکسونومیک گونه Leutea turcomanica از نظر فیلوژنی نیز در جنس مذکور تایید می‌گردد.

Keywords [Persian]

  • ETS
  • Ferula
  • ITS
  • ایران
  • Sect. Merwia
  • گیاهان دارویی
  • فیلوژنی

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The Iranian Journal of Botany
Volume 29, Issue 2
December 2023
Pages 89-105

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History

  • Receive Date: 13 August 2023
  • Revise Date: 27 November 2023
  • Accept Date: 28 November 2023

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