AN OVERVIEW OF THE PHYLOGENETIC RELATIONSHIPS OF BROMUS PUMILIO (POACEAE) AND ALLIES BASED ON nrDNA ITS AND trnL-F SEQUENCES

Document Type : Research Paper

Authors

1 Faculty of Science and Institute of Biological Science, Damghan University, Damghan, Iran.

2 School of Biology and Institute of Biological Science

Abstract

According to various taxonomic treatments, Bromus has been divided into 8 to 9 sections, subgenera or even separate genera. The relationship of Bromus pumilio (syn.: Boissiera squarrosa) with other species of Bromus has long been a matter of dispute. To clarify this problem, the nuclear ribosomal DNA ITS and chloroplast DNA trnL-F sequences for 31 and 28 species respectively were used to reconstruct the phylogenetic relationship and reveal the infrageneric boundaries. Molecular analysis performed by Maximum Parsimony analyses and Bayesian method. The results based on nrDNA ITS show that sects. Bromus, Ceratochloa, Genea, Mexibromus and Neobromus are monophyletic, while the plastid data doesn’t support the monophyly of any sections in the genus Bromus.Evidence based on the nrDNA ITS data doesn’t show any close relationships between B. pumilio (sect. Boissiera) and B. danthoniae or B. lanceolatus (sect. Bromus) and instead robustly supports B. pumilio as the sister group of B. gracillimus (sect. Nevskiella), while in the plastid data B. pumilio is the sister group of a clade comprising B. inermis (sect. Pnigma), B. gunckelii (sect. Neobromus)and species of sect. Ceratochloa.

Keywords

Article Title [Persian]

بررسی اجمالی روابط فیلوژنتیکی Bromus pumilio (Poaceae) و خویشاوندان براساس توالی‌های nrDNA ITS و trnL-F

Authors [Persian]

  • زهرا پورمشیر 1
  • عاطفه امیراحمدی 2
  • رضا نادری 1
1 فارغ التحصیل کارشناسی ارشد، گرایش سیستماتیک-بوم‌شناسی، دانشگاه دامغان
2 دانشکده زیست شناسی و پژوهشکده علوم زیستی، دانشگاه دامغان
Abstract [Persian]

برپایه مطالعات تاکسونومیک متعدد جنس Bromus به 8 تا 9 بخش، زیرجنس و یا جنس‌های مجزا تقسیم‌بندی می‌شود. مدت مدیدی است که رابطه بین گونهBromus pumilio (syn: Boissiera squarrosa)  با گونه‌های دیگر جنس Bromus موضوع بحث است. جهت بازسازی روابط فیلوژنی و آشکارسازی مرزهای درون جنسی از 31 توالی ریبوزومی هسته‌ای ITS و 28 توالی کلروپلاستی trnL-F استفاده شد. تحلیل‌های مولکولی به‌وسیله روش پارسیمونی و بایسین اجرا شد. نتایج برپایه توالی ITS هسته‌ای ریبوزومی نشان می‌دهد که بخش‌های Bromus، Ceratochloea، Genea، Mexibromus و Neobromus تک‌نیا هستند در حالیکه داده‌های کلروپلاستی تک‌نیا بودن هیچ یک از بخش‌های جنس Bromus را نشان نمی‌دهد. شواهد بر پایه داده‌های ریبوزومی ITS هیچ رابطه نزدیکی بین گونه B. pumilio (sect. Boissiera) با گونه‌های B. danthoniae یا B. lanceolatus  (sect. Bromus) نشان نمی‌دهد و به‌جای‌آن گونه B. pumilio را با حمایت بالا به‌عنوان گروه خواهری B. gracillimus (sect. Nevskiella) در نظر می‌گیرد، همچنین در داده‌های کلروپلاستی گونه B. pumilio گروه خواهری کلادی متشکل از B. inermis (sect. Pnigma)، B. gunckelii (sect. Neobromus) و گونه‌های بخش Ceratochloa است.

Keywords [Persian]

  • Bromus pumilio
  • Boissiera
  • phylogeny
  • flora of Iran
  • Poaceae

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The Iranian Journal of Botany
Volume 25, Issue 1
June 2019
Pages 1-10

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History

  • Receive Date: 14 November 2018
  • Revise Date: 26 January 2019
  • Accept Date: 17 April 2019

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