SEED MORPHOLOGY IMPROVES DATA FROM PHYLOGENETIC RELATIONSHIPS OF MEDICAGOID TRIGONELLA, MEDICAGO AND MELILOTUS (FABACEAE) SPECIES

Document Type : Research Paper

Authors

1 Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), P. O. Box 13185-116, Tehran, Iran

3 Islamic Azad University, Science and Research Branch, Tehran, Iran.

Abstract

Phylogenetic relationship and seed morphological characters of 11 species belong to Medicagoid Trigonella, six non Medicagoid and one species of each genera of Medicago and Melilotus were estimated before based on ITS sequences by using Baysian inference and maximum parsimony. In this paper, scanning Electron Microscope and stereomicroscope for seed morphological characters were used. Seed morphology results indicated that majority of Medicagoid Trigonella species belong to Bucerates and Lunatae sections placed in two close subclusters and joined together as did by Baysian analysis results. Both studies confirmed that Medicago radiata is sister for these species. Our results confirm that using molecular and morphological characters data are reliable evidence in systematic discriminations of the taxa.

Keywords

Article Title [Persian]

ریخت‌شناسی دانه اطلاعات قرابت فیلوژنتیک بین گونه‌هایMedicagoid Trigonella و Medicago و Melilotus را با استفاده از مارکرمولکولی ITS تکمیل می نماید

Authors [Persian]

  • سیما خندانی 1
  • مصطفی اسدی 2
  • ایرج مهرگان 3
  • طاهر نژادستاری 3
  • رمضانعلی خاورری نژاد 3
1 دانشکده علوم دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران
2 مؤسسه تحقیقات جنگلها و مراتع کشور
3 واحد علوم و تحقیقات دانشگاه آزاد اسلامی، تهران
Abstract [Persian]

ارتباط فیلوژنتیکی 11 گونه متعلق به Medicagoid Trigonella و 6 گونه متعلق به Trigonella غیر Medicagoid، یک گونه از هر کدام از جنس‌های Medicago و Melilotus با استفاده از توالی منطقه ITS با روش ماکزیمم پارسیمونی وبایسین قبلاً انجام شد. در این مقاله بررسی‌های ریختی دانه توسط میکروسکوپ الکترونی اسکنینگ و استریومیکروسکوپ انجام گرفت. نتایج بدست آمده از ریخت‌شناسی دانه با نتایج حاصل از مطالعات ITS همخوانی دارد و گونه‌های Medicagoid Trigonella در یک دندروگرام با حمایت بالا قرار گرفته و منوفیلتیک هستند. همچنین نتایج مطالعات مورفولوژی دانه قرار گرفتن گونه‌های بخشه‌های Bucerates وLunatea متعلق به گروه Medicagoid Trigonella  در دو زیر خوشه نزدیک به هم را تایید می‌کند. در هردو مطالعه انجام یافته گونه Medicago radiata به عنوان گروه خواهری گونه‌های مورد مطالعه در نظر گرفته شد. این نتایج هماهنگی نتایج مورفولوژیکی و مولکولی را در حل اختلافات تاگزونومیکی بیان می‌دارد.

Keywords [Persian]

  • Fabaceae
  • Medicagoid
  • seed morphology
  • Trigonella
  • Molecular Phylogeny

References

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The Iranian Journal of Botany
Volume 24, Issue 1
July 2018
Pages 45-51

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History

  • Receive Date: 20 May 2017
  • Revise Date: 01 March 2018
  • Accept Date: 23 May 2018

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