GENETIC VARIATION ANALYSIS OF DIFFERENT POPULATIONS OF ROSA DAMASCENA IN NW. IRAN USING RAPD MARKERS

Document Type : Research Paper

Authors

1 Biotechnology Research Department of Natural Resources, Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran.

2 Zanjan Province Natural Resources Office, Zanjan, Iran.

3 Depaetment of Biology, Tehran University, Tehran, Iran.

4 Department of Plant Breeding, Tehran University, Tehran, Iran.

5 Department of Horticultural Science, Tabiat Modares University(TMU), Tehran, Iran.

Abstract

Random amplified polymorphic DNA (RAPD) markers have been used to characterize the genetic diversity among 12 Damask rose landraces from northwest of Iran. The 22 primers used in this study amplified 262 scorable RAPD loci among which 180 were polymorphic (67%). The average number of bands was 11.9 for each primer. Genetic similarity calculated from the RAPD data ranged from 0.261 to 0.995 with an average of 0.63. A dendrogram was prepared on the basis of a similarity matrix using the UPGMA algorithm and separated the 12 landraces into two groups. There was a good correspondence between genetic divergence and climate condition, though some landraces (such as L2 and L3) with diverse geographical condition were clustered in one group. The results showed a possible application of RAPD markers in assessment of genetic diversity in Rosa damascena landraces.

Keywords

Article Title [Persian]

بررسی تنوع ژنتیکی جمعیت های مختلف گل محمدی شمال غرب ایران با استفاده از نشانگر RAPD

Authors [Persian]

  • سید رضا طبائی عقدائی 1
  • حسین حسینی منفرد 2
  • حمید فهیمی 3
  • حسن ابراهیم زاده 3
  • مریم جبلی 1
  • محمدرضا نقوی 4
  • علیرضا بابائی 5
1 گروه تحقیقات بیوتکنولوژی موسسه تحقیقات جنگلها و مراتع، تهران، ایران
2 منابع طبیعی زنجان، زنجان، ایران
3 دانشگاه تهران، تهران، ایران
4 گروه اصلاح نباتات، دانشگاه تهران، تهران، ایران.
5 گروه علوم باغبانی، دانشگاه تربیت مدرس، تهران، ایران.
Abstract [Persian]

در این پژوهش نشانگر مولکولیRAPD برای تعیین تنوع ژنتیکی 12 جمعیت گل محمدی شمال غرب ایران مورد استفاده قرار گرفت. با استفاده از 22 آغازگر، تعداد 262 باند قابل تشخیص ایجاد شد که 67% آنها ( 180 باند) در بین جمعیتهای مختلف چند شکلی نشان دادند. تعداد متوسط باندها به ازای هر آغازگر 9/11 بود. شاخص تشابه Dice برای تعیین همسانیهای ژنتیکی بین جمعیتها مورد استفاده قرار گرفته و با استفاده از الگوریتم UPGMA یک دندروگرام بر مبنای ماتریس تشابه تهیه شد که 12 جمعیت گل محمدی را در2 گروه مختلف قرار داد. نتایج بدست آمده ارتباط خوبی بین تنوع آب و هوایی و مولکولی نشان دادند، گرچه جمعیتهای با تنوع جغرافیایی (مانند L2 وL3) نیز در یک گروه قرار گرفتند. نتایج این پژوهش بر امکان بکارگیری نشانگرهای RAPD در ارزیابی تنوع ژنتیکی ژرم پلاسم گل محمدی تاکید داشت.

Keywords [Persian]

  • Rosa damascena Mill
  • genetic similarity
  • RAPD analysis

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The Iranian Journal of Botany
Volume 12, Issue 2 - Serial Number 2
December 2006
Pages 121-127

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History

  • Receive Date: 28 October 2015
  • Accept Date: 28 October 2015

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