TRANSFERABILITY AND POLYMORPHISM OF BARLEY MICROSATELLITE MARKERS ACROSS H-GENOME CONTAINING SPECIES IN THE GENUS HORDEUM (H. VULGARE AND H. BULBOSUM)

Document Type : Research Paper

Authors

1 PhD Student / Department of Biology, University of Isfahan, Isfahan, Iran

2 Assistant Professor / Department of Biology, University of Isfahan, Isfahan, Iran

3 Professor / Department of Biology, University of Isfahan, Isfahan, Iran

4 Professor / Crop Genome Research Unit, National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

Abstract

Limited numbers of microsatellite markers are available for genetic characterization of Hordeum bulbosum which comprises the secondary genepool of cultivated barely. The objective of this study was to evaluate the transferability of microsatellite markers from H. vulgare to H. bulbosum and a preliminary evaluation of their polymorphism. From ninety-three pairs barley SSR primer tested for transferability, all of them  amplified DNA segments in H. vulgare (11 accessions) and48 pairs (51.61%) were transferable to the H. bulbosum (5 accessions) with high level of polymorphism. Twenty-two (23.65%) SSR markers showed transferability to H. murinum used as outgroup. A total of 546 alleles were detected by 48 transferred primer pairs in all accessions. The number of alleles per locus ranged from 3 to 13 with an average of 11.375 alleles per locus. The PICvalues were ranged from 0.161 to 0.621 with an average of 0.477. The value of PIC in H. vulgare (average PIC = 0.639) was significantly higher than H. bulbosum (average PIC = 0.316). In dendrogram generated based on SSR data accessions were divided into groups related to their taxonomic classifications, indicating the efficiency of barley SSRs for phylogenetic analyses in H genome containing species in the genus Hordeum. Based on the results of this study, it can be suggested that the cross species transferable barley SSRs are valuable molecular tools, for genetic diversity analyses in the H. bulbosum for which limited number of microsatellite markers are available. This study provided a set of efficient SSR markers from publicly available barley microsatellite markers for the genetic characterization of H. bulbosum.

Keywords


Article Title [Persian]

قابلیت انتقال و تنوع ریزماهوارک‌های جو زراعی به گونه‌های واجد ژنوم H در جنس جو (Hordeum vulgare, H. bulbosum)

Authors [Persian]

  • حامد خدایاری 1
  • حجت‌الله سعیدی 2
  • محمدرضا رحیمی‌نژاد 3
  • تاکائو کوماتسودا 4
1 دانشجوی دکتری / گروه زیست‌شناسی دانشگاه اصفهان
2 استادیار / گروه زیست‌شناسی دانشگاه اصفهان
3 استاد / گروه زیست‌شناسی دانشگاه اصفهان
4 استاد / مرکز تحقیقات ژنومی گیاهان زراعی، موسسه علوم زیستی کشاورزی (NIAS)، سوکوبا، ژاپن
Abstract [Persian]

تعداد محدودی مارکرهای ریزماهوارک مناسب برای بررسی تنوع وراثتی گونه‌ی H. bulbosumوجود دارد. در این مطالعه قابلیت انتقال ریزماهوارک‌های جو زراعی به گونه‌ی H. bulbosumو تنوع پذیری آنها مورد بررسی قرار گرفته است. از 93 زوج پرایمر SSR آزمایش شده همگی در گونه‌ی H. vulgare(11 نمونه بذر) قطعاتی از DNA را تکثیر نمودند ولی تنها 46 زوج از آنها (61/51%) قابلیت انتقال به گونه H. bulbosum (5 نمونه بذر) با سطح قابل ملاحظه‌ای از تنوع را نشان دادند. در گونه‌ی  H. murinum که به عنوان برون گروه استفاده شده بود 22 زوج پرایمر SSR (65/23%) قابلیت انتقال نشان دادند. در PCR انجام شده با استفاده از 48 مارکر SSR تعداد 546 آلل در کل جمعیت‌ها تشخیص داده شد. تعداد آلل‌ها در هر جایگاه وراثتی SSR بین 3 تا 13 با میانگین 375/11 آلل بود. مقدار PIC محاسبه شده برای هر مارکر بین 161/0 تا 621/0 با میانگین 477/. بود. مقدار PIC در گونه‌ی H. vulgare (با میانگین 639/0) به طور معنی داری بالاتر از H. bulbosum(با میانگین 316/0) بود. در دندروگرام حاصل جمعیت‌ها بر اساس گروه های تاکسونومیک از هم جدا شدند که نشان‌دهنده‌ی کارآیی این مارکرها برای مطالعه فیلوژنی درون این گروه است. نتایج این مطالعه نشان می‌دهد که مایکروساتلایت‌های جو زراعی برای بررسی تنوع وراثتی گونه‌ی H. bulbosumمناسب هستند.
 

Keywords [Persian]

  • Microsatellites
  • Genetic diversity
  • Breeding
  • Transferability
  • H genome
  • Barley
  • germplasm
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